Human sperm rotate with a conserved direction during free swimming in four dimensions
Journal of Cell Science,
Journal Year:
2023,
Volume and Issue:
136(22)
Published: Oct. 30, 2023
Head
rotation
in
human
spermatozoa
is
essential
for
different
swimming
modes
and
fertilisation,
as
it
links
the
molecular
workings
of
flagellar
beat
with
sperm
motion
three-dimensional
(3D)
space
over
time.
Determining
direction
head
has
been
hindered
by
symmetry
translucent
nature
head,
fast
3D
driven
helical
beat.
Analysis
mostly
restricted
to
two-dimensional
(2D)
single
focal
plane
image
analysis,
which
enables
tracking
centre
position
but
not
rotation.
Despite
conserved
beating
flagellum,
reported
be
uni-
or
bi-directional,
even
intermittently
change
a
given
cell.
Here,
we
directly
measure
freely
using
multi-plane
4D
(3D+t)
microscopy
show
that:
(1)
2D
unable
distinguish
spermatozoa;
(2)
non-capacitating
capacitating
solutions,
both
aqueous
viscous
media,
counterclockwise
(CCW),
seen
from
tail,
all
rotating
spermatozoa,
regardless
experimental
conditions;
(3)
suppressed
36%
medium,
although
CCW
recovered
after
incubation
conditions
within
same
possibly
unveiling
an
unexplored
aspect
need
capacitation
fertilisation.
Our
observations
that
conserved.
It
constitutes
robust
persistent
driving
mechanism
influences
navigation
time,
thus
critical
importance
cell
motility,
propulsion
flagellated
microorganisms,
motility
assessments,
reproduction
research,
self-organisation
patterns
space.
Language: Английский
Bull sperm cryopreservation: An overview on the current status and future perspectives
German Journal of Veterinary Research,
Journal Year:
2024,
Volume and Issue:
4(1), P. 9 - 22
Published: Jan. 1, 2024
Cryopreservation
refers
to
freezing
cells
or
tissues
at
extremely
low
temperatures,
allowing
them
be
stored
for
extended
periods
while
maintaining
viability.
Cryopreserved
bull
semen
has
become
an
essential
tool
in
cattle
breeding
programs
and
commercial
production
systems.
This
review
provides
a
detailed
analysis
of
the
current
methods
challenges
preserving
sperm
using
cryopreservation.
We
explore
effects
cryopreservation
on
cells,
role
different
cryoprotectants,
as
well
progress
made
semen.
It
also
highlights
impact
process
morphology
functionality,
emphasizing
importance
optimizing
techniques
maintain
fertility
The
article
underscores
significance
technology
genetics
suggests
future
research
enhance
techniques.
Language: Английский