Predicting the unpredictable: the regulatory nature and promiscuity of herbicide cross resistance
Pest Management Science,
Journal Year:
2023,
Volume and Issue:
80(2), P. 235 - 244
Published: Aug. 18, 2023
Abstract
The
emergence
of
herbicide‐resistant
weeds
is
a
significant
threat
to
modern
agriculture.
Cross
resistance,
phenomenon
where
resistance
one
herbicide
confers
another,
particular
concern
owing
its
unpredictability.
Nontarget‐site
(NTS)
cross
especially
challenging
predict,
as
it
arises
from
genes
that
encode
enzymes
do
not
directly
involve
the
target
site
and
can
affect
multiple
herbicides.
Recent
advancements
in
genomic
structural
biology
techniques
could
provide
new
venues
for
predicting
NTS
weed
species.
In
this
review,
we
present
an
overview
latest
approaches
be
used.
We
discuss
use
epigenomics
such
ATAC‐seq
DAP‐seq
identify
transcription
factors
cis‐regulatory
elements
associated
with
traits.
Enzyme/protein
structure
prediction
docking
analysis
are
discussed
initial
step
binding
affinities
key
candidates
subsequent
vitro
validation.
also
example
analyses
deployed
toward
elucidating
regulatory
patterns.
Ultimately,
our
review
provides
important
insights
into
scientific
potential
directions
managing
weeds.
©
2023
Authors.
Pest
Management
Science
published
by
John
Wiley
&
Sons
Ltd
on
behalf
Society
Chemical
Industry.
Language: Английский
Missing Genomic Resources for the Next Generation of Environmental Risk Assessment
Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(4), P. 1877 - 1881
Published: Jan. 21, 2024
Environmental
risk
assessment
traditionally
relies
on
a
wide
range
of
in
vivo
testing
to
assess
the
potential
hazards
chemicals
environment.
These
tests
are
often
time-consuming
and
costly
can
cause
test
organisms'
suffering.
Recent
developments
reliable
low-cost
alternatives,
both
vivo-
silico-based,
opened
door
reconsider
current
toxicity
assessment.
However,
many
these
new
approach
methodologies
(NAMs)
rely
high-quality
annotated
genomes
for
surrogate
species
regulatory
Currently,
lack
genomic
information
slows
process
NAM
development.
Here,
we
present
phylogenetically
resolved
overview
missing
resources
within
ecotoxicological
We
call
an
organized
systematic
effort
(regulatory)
community
provide
resources.
Further,
discuss
standardized
landscape
enable
robust
nonanimal-reliant
systems
ecotoxicology
era.
Language: Английский
Enhanced metabolic detoxification is associated with fluroxypyr resistance in Bassia scoparia
Plant Direct,
Journal Year:
2024,
Volume and Issue:
8(1)
Published: Jan. 1, 2024
Abstract
Auxin‐mimic
herbicides
chemically
mimic
the
phytohormone
indole‐3‐acetic‐acid
(IAA).
Within
auxin‐mimic
herbicide
class,
fluroxypyr
has
been
extensively
used
to
control
kochia
(
Bassia
scoparia
).
A
2014
field
survey
for
resistance
in
populations
across
Colorado
identified
a
putative
fluroxypyr‐resistant
(Flur‐R)
population
that
was
assessed
response
and
dicamba
(auxin‐mimics),
atrazine
(photosystem
II
inhibitor),
glyphosate
(EPSPS
chlorsulfuron
(acetolactate
synthase
inhibitor).
This
resistant
but
sensitive
glyphosate,
atrazine,
dicamba.
Subsequent
dose‐response
studies
determined
Flur‐R
40
times
more
than
susceptible
(J01‐S)
collected
from
same
(LD
50
720
20
g
ae
ha
−1
,
respectively).
Auxin‐responsive
gene
expression
increased
following
treatment
Flur‐R,
J01‐S,
dicamba‐resistant,
fluroxypyr‐susceptible
line
9,425
an
RNA‐sequencing
experiment.
In
several
transcripts
with
molecular
functions
conjugation
transport
were
constitutively
higher
expressed,
such
as
glutathione
S‐transferases
(GSTs),
UDP‐glucosyl
transferase
(GT),
ATP
binding
cassette
transporters
(ABC
transporters).
After
analyzing
metabolic
profiles
over
time,
both
J01‐S
rapidly
converted
[
14
C]‐fluroxypyr
ester,
formulation
applied
plants,
acid,
biologically
active
form
of
herbicide,
three
unknown
metabolites.
The
formation
flux
these
metabolites
faster
reducing
concentration
phytotoxic
acid.
One
unique
metabolite
present
not
profile.
Gene
sequence
variant
analysis
specifically
auxin
receptor
signaling
proteins
revealed
absence
non‐synonymous
mutations
affecting
candidate
target
site
genes,
further
supporting
our
hypothesis
non‐target
degradation
is
contributing
Flur‐R.
Significance
Statement:
Herbicide
ever‐present
issue
weeds
cropping
rangeland
systems.
Every
characterized
mechanism
contributes
working
database
address
agricultural
or
open‐space
setting.
Knowing
exact
helps
public
sector
industry
researchers
understand
why
applications
are
failing,
if
plants
can
still
be
controlled
other
modes
action.
there
strong
evidence
support
mechanism,
via
enzymatic
activity.
Increased
represents
novel
.
Language: Английский
A phased chromosome-level genome assembly provides insights into the evolution of sex chromosomes inAmaranthus tuberculatus
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 2, 2024
Summary
Amaranthus
tuberculatus
(waterhemp)
is
a
troublesome
weed
species
of
agronomic
importance
that
dioecious
with
an
XY
sex-determination
system.
The
evolution
sex
chromosomes,
the
contiguity
sex-determining
region
(SDR)
and
expression
pattern
genes
within
SDR
remain
poorly
understood.
We
assembled
genome
male
A.
,
phased
into
two
chromosome-level
haplotypes,
performed
restriction
site-associated
DNA
genome-
wide
association
(RAD-GWA)
analysis,
comparative
genomics,
adaptive
and,
existing
data,
transcriptomic
profiling
to
characterize
species’
chromosomes.
Comparative
analysis
enabled
identification
∼32.8
Mb
on
chromosome
1
gene-poor,
abundant
in
long
terminal
repeat
(LTR)
retrotransposons,
harbors
inversions.
Synteny
revealed
likely
originated
from
fusion
ancestral
mRNA
data
indicated
93
out
531
protein-coding
haplome
2
were
differentially
expressed
between
mature
female
flowers,
several
enriched
for
Gene
Ontology
(GO)
terms
involved
floral
development.
Beyond
adding
our
understanding
evolution,
genomic
resource
provided
here
will
be
valuable
addressing
further
questions
trait
.
Language: Английский
Unraveling the Role of P450 Reductase in Herbicide Metabolic Resistance Mechanism
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 20, 2023
SUMMARY
Plants
require
cytochrome
P450
reductase
(CPR)
to
supply
two
electrons
for
monooxygenase
enzymes
(P450)
react
with
an
organic
substrate.
The
transfer
of
the
active
site
in
catalytic
relies
on
a
robust
and
intricate
CPR:P450
complex
endoplasmic
reticulum
membrane.
Transgenic
Arabidopsis
plants
carrying
CYP81A12
from
Echinochloa
phyllopogon
,
which
metabolize
broad
spectrum
herbicides,
were
crossed
CPR
knockout
atr1
or
atr2
mutant
lines.
Homozygous
gene
was
confirmed
using
PCR,
copy
number
determined
ddPCR.
lines
expressing
combination
used
herbicide
dose-response
metabolism
studies.
Knocking
out
ATR1
transgenic
significantly
reduced
resistance.
(
atr1-b
)
had
3.6-,
5.6-,
6.8-
at
least
26-
fold
reduction
resistance
mesotrione,
2,4-D,
penoxsulam
chlorsulfuron,
respectively,
assay.
Knockouts
ATR2
also
decreased
resistance,
but
lower
magnitude
than
.
These
results
through
½
MS
medium
assay,
observed
additional
tested
bensulfuron,
propoxycarbazone
bentazon.
Our
findings
highlight
importance
CPRs
metabolic
plants,
specifically
identifying
that
is
most
important
metabolism.
different
found
weeds
have
potential
as
target
genes
manage
evolution.
We
further
provide
in-depth
exploration
evolutionary
implications
weed
management
arising
results.
Language: Английский
Enhanced metabolic detoxification is associated with fluroxypyr resistance inBassia scoparia
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Aug. 31, 2023
Abstract
Auxin-mimic
herbicides
chemically
mimic
the
phytohormone
indole-3-acetic-acid
(IAA).
Within
auxin-mimic
herbicide
class,
fluroxypyr
has
been
extensively
used
to
control
an
agronomically
problematic
Great
Plains
tumbleweed,
kochia
(
Bassia
scoparia
).
A
2014
field
survey
for
resistance
in
populations
across
Colorado
identified
a
putative
resistant
population
that
was
assessed
response
five
different
representing
four
modes
of
action.
These
included
and
dicamba
(auxin-mimics),
atrazine
(photosystem
II
inhibitor),
glyphosate
(EPSPS
chlorsulfuron
(acetolactate
synthase
inhibitor).
The
greenhouse
screen
this
chlorsulfuron,
but
sensitive
glyphosate,
atrazine,
dicamba.
This
designated
Flur-R.
Subsequent
dose
studies
determined
75%
Flur-R
survived
628
g
ae
ha
-1
(4X
label
application
rate
wheat
fallow,
which
is
157
at
1X).
40
times
more
than
susceptible
(J01-S)
collected
from
same
(LD
50
720
20
,
respectively).
Auxin-responsive
gene
expression
increased
following
treatment
Flur-R,
J01-S,
dicamba-resistant,
fluroxypyr-susceptible
line
9425
RNA-sequencing
experiment.
In
several
transcripts
with
molecular
functions
conjugation
transport
were
constitutively
higher
expressed,
such
as
glutathione
S-transferases
(GSTs),
UDP-glucosyl
transferase
(GT),
ATP
binding
cassette
transporters
(ABC
transporters).
After
analyzing
metabolic
profiles
over
time,
both
J01-S
rapidly
converted
[
14
C]-fluroxypyr
ester,
formulation
applied
plants,
acid,
biologically
active
form
herbicide,
three
unknown
metabolites.
Formation
flux
these
metabolites
faster
reducing
concentration
phytotoxic
acid.
One
unique
metabolite
present
not
profile.
Gene
sequence
variant
analysis
specifically
auxin
receptor
signaling
proteins
revealed
absence
non-synonymous
mutations
affecting
candidate
target
site
genes,
further
supporting
our
hypothesis
non-target
degradation
contributing
Significance
Statement
Herbicide
ever-present
issue
weeds
cropping
rangeland
systems.
By
understanding
genetic
mechanisms
individual
cases
resistance,
we
can
extrapolate
important
information
how
quickly
specific
spread.
Every
characterized
mechanism
contributes
working
database
address
agricultural
or
open-space
setting.
Knowing
exact
helps
researchers
industry
members
understand
why
applications
are
failing,
if
plants
still
be
controlled
other
there
strong
evidence
support
mechanism,
via
enzymatic
activity.
Increased
represents
novel
weed
.
Language: Английский
Missing genomic resources for the next generation of environmental risk assessment
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Oct. 14, 2023
Abstract
Environmental
risk
assessment
traditionally
relies
on
a
wide
range
of
in
vivo
testing
to
assess
the
potential
hazard
chemicals
environment.
These
tests
are
often
time-consuming,
costly
and
can
cause
test
organisms’
suffering.
Recent
developments
reliable
low-cost
alternatives,
both
-
silico
-based,
opened
door
reconsider
current
toxicity
assessment.
However,
many
these
new
approach
methodologies
(NAMs)
rely
high
quality
annotated
genomes
for
surrogate
species
regulatory
Currently,
lacking
genomic
information
slows
process
NAM
development.
Here,
we
present
phylogenetically
resolved
overview
missing
resources
within
ecotoxicological
We
call
an
organized
systematic
effort
(regulatory)
community
provide
resources.
Further,
discuss
standardized
landscape
enable
robust
non-animal
reliant
systems
ecotoxicology
era.
Synopsis
Statement
identify
needed
development
acceptance
environmental
Language: Английский