‘One Health’ Genomic Surveillance of Avian and Human Influenza A Viruses Through Environmental Wastewater Monitoring DOI Open Access
Andrew Lee,

Stephen Carson,

Marina Reyne

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 13, 2023

Abstract Background Influenza A viruses (IAV) are significant pathogens of humans and other animals. Although endemic in birds, novel IAV strains can emerge, jump species, cause epidemics, like the latest variant H5N1. Wastewater-based epidemiology (WBE) has very recently been shown to detect human but whether it avian-origin IAV, if whole genome sequencing (WGS) be used discriminate circulating wastewater remains unknown. Methods Using a pan-IAV RT-qPCR assay, six treatment works (WWTWs) across Northern Ireland (NI), were screened from August December 2022. WGS approach using Oxford Nanopore technology was employed sequence positive samples. Phylogenetic analysis sequences relative currently avian IAVs performed. Findings We detected dynamic signal September 2022 onwards NI. “Meta” generated displaying homology both strains. The proportion versus reads differed time sample site. diversity subtypes lineages (e.g. H1N1, H3N2, several avian). Avian segment 8 related those found recent H5N1 clade 2.3.4.4b identified. Interpretation WBE affords means monitor provide crucial genetic information. As such rapid, cost-effective, year-round “one-health” surveillance help control epidemic pandemic threats. Funding This study funded by Department Health for as part Wastewater Surveillance Programme. Highlights Dynamic Nanopore-based reveals presence wastewater. similarity gull-associated H13/H16 isolates. Co-detection distinct clades H1N1 H3N2 subtypes. Author Summary virus is major pathogen animals causes regular epidemics devastating pandemics. Recently, highly-pathogenic influenza emerged spreading world killing millions birds infecting mammals, enhancing its potential. Strengthening global systems animal thus priority. applied track SARS-CoV-2 this could work not known. Here, we develop method survey levels genetically characterise viruses. Through highlight co-detection wastewater, with Our demonstrates potential defend against only infections emerging, zoonotic Graphical

Language: Английский

Defining alarm thresholds for the load of pathogenic viruses in wastewater for decision making: An application to three French cities DOI
Olivier Schlosser, Sophie Courtois,

Philippe Bryche

et al.

International Journal of Hygiene and Environmental Health, Journal Year: 2025, Volume and Issue: 266, P. 114563 - 114563

Published: March 15, 2025

Language: Английский

Citations

0
Spatial and temporal variation in respiratory syncytial virus (RSV) subtype RNA in wastewater and relation to clinical specimens DOI Creative Commons

Winnie Zambrana,

ChunHong Huang,

D. Solis

et al.

mSphere, Journal Year: 2024, Volume and Issue: 9(7)

Published: June 27, 2024

Respiratory syncytial virus (RSV) causes a large burden of respiratory illness globally. It has two subtypes, RSV A and B, but little is known regarding the predominance these subtypes during different seasons their impact on morbidity mortality. Using molecular methods, we quantified B RNA in wastewater solids across multiple metropolitan areas to gain insight into subtypes. We determined predominant subtype for each group using proportion total (RSV + B) sample (

Language: Английский

Citations

3
Wastewater monitoring of human and avian influenza A viruses in Northern Ireland: a genomic surveillance study DOI Creative Commons
Andrew Lee,

Stephen Carson,

Marina Reyne

et al.

The Lancet Microbe, Journal Year: 2024, Volume and Issue: unknown, P. 100933 - 100933

Published: Oct. 1, 2024

Influenza A viruses (IAVs) are significant pathogens of humans and other animals. Although endemic in birds, novel IAV strains can emerge, jump species, cause epidemics, like the latest variant H5N1. Wastewater-based epidemiology (WBE) has been shown capable detecting human IAVs. We aimed to assess whether whole-genome sequencing (WGS) IAVs from wastewater is possible be used discriminate between circulating any non-human IAVs, such as those avian origin.

Language: Английский

Citations

2
Correlating Quantitative and Genomic SARS-CoV-2 Wastewater Data with Clinical Metrics in Metropolitan Perth, Western Australia DOI Open Access
Jake Gazeley, Terence Lee, Daniel R. Knight

et al.

Published: March 5, 2024

Wastewater-based epidemiology (WBE) has emerged as a key method for continuous monitoring of COVID-19 prevalence including circulating SARS-CoV-2 lineages. WBE addresses limitations traditional clinical surveillance such test availability, fluctuating testing rates and increased reliance on rapid antigen tests. Our study in Perth, Western Australia, found significant positive correlation between concentrations wastewater PCR positivity (rs = 0.772; p < 0.001) over an 18-month period that included four successive waves. A strong was apparent the proportions lineages cases within same region 0.728; 0.001), earlier detection Omicron recombinant before case confirmation. The successful integration with healthcare data underscores its critical role enhancing public health decision-making pandemic management. This approach not only demonstrates value current global efforts but also highlights potential to address future challenges, comprehensive disease response approach.

Language: Английский

Citations

1
Spatial and temporal variation in respiratory syncytial virus (RSV) subtype RNA in wastewater, and relation to clinical specimens DOI Open Access

Winnie Zambrana,

ChunHong Huang,

D. Solis

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 18, 2024

Abstract Respiratory syncytial virus (RSV) causes a large burden of respiratory illness, globally. It has two subtypes, RSV A and B, but little is known regarding the predominance these subtypes during different seasons their impact on morbidity mortality. Using molecular methods, we quantified B RNA in wastewater solids across multiple metropolitan areas to gain insight into subtypes. We determined predominant subtype for each group using proportion total (RSV + B) sample (P A,WW ), conducted comparative analysis temporally, spatially against clinical specimens. median P 0.00 first season 0.58 second indicated temporal shift subtype. Spatially, while observed dominance same subtype, was higher some = 0.88). The predominated samples, samples showed levels positivity 79%, 0.58). These results suggest that wastewater, alongside data, holds promise enhanced surveillance. Importance study illustrates information can be gleaned from wastewater. As biological composite entire contributing population, monitoring complement surveillance RSV.

Language: Английский

Citations

0
Correlating Quantitative and Genomic SARS-CoV-2 Wastewater Data with Clinical Metrics in Metropolitan Perth, Western Australia DOI Open Access
Jake Gazeley, Terence Lee, Daniel R. Knight

et al.

Environments, Journal Year: 2024, Volume and Issue: 11(4), P. 62 - 62

Published: March 24, 2024

Wastewater-based epidemiology (WBE) has emerged as a key method for the continuous monitoring of COVID-19 prevalence including circulating SARS-CoV-2 lineages. WBE addresses limitations traditional clinical surveillance such test availability, fluctuating testing rates, and increased reliance on rapid antigen tests. Our study in Perth, Western Australia found significant positive correlation between concentrations wastewater PCR positivity rates (rs = 0.772; p < 0.001) over an 18-month period that included four successive waves. A strong was apparent proportions lineages cases within same region 0.728, 0.001), earlier detection Omicron recombinant before case confirmation. The successful integration with healthcare data underscores its critical role enhancing public health decision-making pandemic management. This approach not only demonstrates value current global efforts but also highlights potential to address future challenges, comprehensive disease response approach.

Language: Английский

Citations

0
Sewage surveillance revealed the seasonality and prevalence of respiratory syncytial virus and its implications for seasonal immunization strategy in low and middle-income regions of China DOI
Ziqiang Zhang, Qingxiang Li,

Fenglan He

et al.

Water Research, Journal Year: 2024, Volume and Issue: 270, P. 122828 - 122828

Published: Nov. 22, 2024

Language: Английский

Citations

0
‘One Health’ Genomic Surveillance of Avian and Human Influenza A Viruses Through Environmental Wastewater Monitoring DOI Open Access
Andrew Lee,

Stephen Carson,

Marina Reyne

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 13, 2023

Abstract Background Influenza A viruses (IAV) are significant pathogens of humans and other animals. Although endemic in birds, novel IAV strains can emerge, jump species, cause epidemics, like the latest variant H5N1. Wastewater-based epidemiology (WBE) has very recently been shown to detect human but whether it avian-origin IAV, if whole genome sequencing (WGS) be used discriminate circulating wastewater remains unknown. Methods Using a pan-IAV RT-qPCR assay, six treatment works (WWTWs) across Northern Ireland (NI), were screened from August December 2022. WGS approach using Oxford Nanopore technology was employed sequence positive samples. Phylogenetic analysis sequences relative currently avian IAVs performed. Findings We detected dynamic signal September 2022 onwards NI. “Meta” generated displaying homology both strains. The proportion versus reads differed time sample site. diversity subtypes lineages (e.g. H1N1, H3N2, several avian). Avian segment 8 related those found recent H5N1 clade 2.3.4.4b identified. Interpretation WBE affords means monitor provide crucial genetic information. As such rapid, cost-effective, year-round “one-health” surveillance help control epidemic pandemic threats. Funding This study funded by Department Health for as part Wastewater Surveillance Programme. Highlights Dynamic Nanopore-based reveals presence wastewater. similarity gull-associated H13/H16 isolates. Co-detection distinct clades H1N1 H3N2 subtypes. Author Summary virus is major pathogen animals causes regular epidemics devastating pandemics. Recently, highly-pathogenic influenza emerged spreading world killing millions birds infecting mammals, enhancing its potential. Strengthening global systems animal thus priority. applied track SARS-CoV-2 this could work not known. Here, we develop method survey levels genetically characterise viruses. Through highlight co-detection wastewater, with Our demonstrates potential defend against only infections emerging, zoonotic Graphical

Language: Английский

Citations

0