Advanced airborne eDNA sampling allows robust spatiotemporal characterisation of vertebrate communities DOI Creative Commons
Kasun H. Bodawatta,

Agnete le Maire Madsen,

Luke E. Holman

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Abstract Effective biodiversity survey methods are crucial for monitoring ecosystems threatened by climatic fluctuations and anthropogenic pressures. Here we advance the collection of a novel source data - airborne environmental DNA (eDNA) investigate whether it yields habitat- season-specific signatures terrestrial vertebrate communities. Using portable low budget samplers, sampled eDNA in three protected nature areas across Denmark show that air filters with large surface area widest range particle retention sizes yield detections higher numbers taxa community homogeneity samples. Further, increased airflow rate dry storage − 20°C more diverse consistent Overall, demonstrate robust spatio-temporal dynamics classes, which can be adapted conservation programs to monitor globe.

Language: Английский

eProbe: Sampling of Environmental DNA within Tree Canopies with Drones DOI
Steffen Kirchgeorg, Jia Jin Marc Chang, Yin Cheong Aden Ip

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(37), P. 16410 - 16420

Published: Sept. 5, 2024

Environmental DNA (eDNA) analysis is a powerful tool for studying biodiversity in forests and tree canopies. However, collecting representative eDNA samples from these high complex environments remains challenging. Traditional methods, such as surface swabbing or rolling, are labor-intensive require significant effort to achieve adequate coverage. This study proposes novel approach unmanned aerial vehicles (UAVs) collect within canopies by using technique. The method involves lowering probe hovering UAV into the canopy it descends ascends through branches leaves. To this, custom-designed robotic system was developed featuring winch collection. design of optimized, control logic reduce risk entanglement while ensuring sufficient interaction force facilitate transfer onto probe. effectiveness this demonstrated during XPRIZE Rainforest Semi-Finals 10 were collected rainforest canopy, total 152 molecular operational taxonomic units (MOTUs) identified metabarcoding. We further investigate how number interactions with vegetation, penetration depth, sampling duration influence concentration community composition samples.

Language: Английский

Citations

4
Advanced airborne eDNA sampling allows robust spatiotemporal characterisation of vertebrate communities DOI Creative Commons
Kasun H. Bodawatta,

Agnete le Maire Madsen,

Luke E. Holman

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Abstract Effective biodiversity survey methods are crucial for monitoring ecosystems threatened by climatic fluctuations and anthropogenic pressures. Here we advance the collection of a novel source data - airborne environmental DNA (eDNA) investigate whether it yields habitat- season-specific signatures terrestrial vertebrate communities. Using portable low budget samplers, sampled eDNA in three protected nature areas across Denmark show that air filters with large surface area widest range particle retention sizes yield detections higher numbers taxa community homogeneity samples. Further, increased airflow rate dry storage − 20°C more diverse consistent Overall, demonstrate robust spatio-temporal dynamics classes, which can be adapted conservation programs to monitor globe.

Language: Английский

Citations

0