An extended and improved CCFv3 annotation and Nissl atlas of the entire mouse brain DOI Creative Commons
Sébastien Piluso, Csaba Verasztó, Harry Carey

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

ABSTRACT Brain atlases are essential for quantifying cellular composition in mouse brain regions. The Allen Institute’s Common Coordinate Framework version 3 (CCFv3) is widely used, delineating over 600 anatomical regions, but it lacks coverage the most rostral and caudal parts, including main olfactory bulb, cerebellum, medulla. Additionally, CCFv3 omits key cerebellar layers, its corresponding Nissl-stained reference volume not precisely aligned, limiting utilisability. To address these issues, we developed an extended atlas, Blue Project augmented (CCFv3aBBP), which includes a fully annotated improved Nissl aligned CCFv3. This enhanced atlas also features central nervous system annotation (CCFv3cBBP). Using this resource, 734 brains to produce average template, enabling updated distribution of neuronal soma positions. These data available as open-source broadening applications such alignment precision, cell type mapping, multimodal integration.

Language: Английский

BlueRecording: A pipeline for the efficient calculation of extracellular recordings in large-scale neural circuit models DOI Open Access
Joseph Tharayil, Jorge Blanco Alonso, Silvia Farcito

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 14, 2024

Abstract As the size and complexity of network simulations accessible to computational neuroscience grows, new avenues open for research into extracellularly recorded electric signals. Biophysically detailed permit identification biological origins different components signals, evaluation signal sensitivity anatomical, physiological, geometric factors, selection recording parameters maximize information content. Simultaneously, virtual extracellular signals produced by these networks may become important metrics neuro-simulation validation. To enable efficient calculation from large neural simulations, we have developed BlueRecording , a pipeline consisting standalone Python code, along with extensions Neurodamus simulation control application, CoreNEURON computation engine, SONATA data format, online such In particular, implement general form reciprocity theorem, which is capable handling non-dipolar current sources, as be found in long axons recordings close source, well complex tissue anatomy, dielectric heterogeneity, electrode geometries. our knowledge, this first application generalized (i.e., non-dipolar) reciprocity-based approach simulate EEG recordings. We use tools calculate an silico model rat somatosensory cortex hippocampus study contribution differences between regions cell types.

Language: Английский

Citations

5

Modeling and Simulation of Neocortical Micro- and Mesocircuitry. Part I: Anatomy DOI Creative Commons
Michael W. Reimann, Sirio Bolaños‐Puchet, Jean-Denis Courcol

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Aug. 15, 2022

Abstract The function of the neocortex is fundamentally determined by its repeating microcircuit motif, but also rich, interregional connectivity. We present a data-driven computational model anatomy non-barrel primary somatosensory cortex juvenile rat, integrating whole-brain scale data while providing cellular and subcellular specificity. consists 4.2 million morphologically detailed neurons, placed in digital brain atlas. They are connected 14.2 billion synapses, comprising local, mid-range extrinsic delineated limits determining connectivity from neuron morphology placement, finding that it reproduces targeting Sst+ requires additional specificity to reproduce PV+ VIP+ interneurons. Globally, was characterized local clusters tied together through hub neurons layer 5, demonstrating how interegional complicit, inseparable networks. suitable for simulation-based studies, 211,712 subvolume made openly available community.

Language: Английский

Citations

8

Large-Scale Mechanistic Models of Brain Circuits with Biophysically and Morphologically Detailed Neurons DOI
Salvador Durá-Bernal, Beatriz Herrera, Carmen Alina Lupaşcu

et al.

Journal of Neuroscience, Journal Year: 2024, Volume and Issue: 44(40), P. e1236242024 - e1236242024

Published: Oct. 2, 2024

Understanding the brain requires studying its multiscale interactions from molecules to networks. The increasing availability of large-scale datasets detailing circuit composition, connectivity, and activity is transforming neuroscience. However, integrating interpreting this data remains challenging. Concurrently, advances in supercomputing sophisticated modeling tools now enable development highly detailed, biophysical models. These mechanistic models offer a method systematically integrate experimental data, facilitating investigations into structure, function, disease. This review, based on Society for Neuroscience 2024 MiniSymposium, aims disseminate recent broader community. It highlights (1) examples current various regions developed through integration; (2) their predictive capabilities regarding cellular mechanisms underlying recordings (e.g., membrane voltage, spikes, local-field potential, electroencephalography/magnetoencephalography) function; (3) use simulating biomarkers diseases like epilepsy, depression, schizophrenia, Parkinson's, aiding understanding underpinnings developing novel treatments. review showcases state-of-the-art covering hippocampus, somatosensory, visual, motor, auditory cortical, thalamic circuits across species. predict neural at multiple scales provide insights sensation, motor behavior, signals, coding, disease, pharmacological interventions, stimulation. Collaboration with neuroscientists clinicians essential validation these models, particularly as grow. Hence, foster interest detailed leading cross-disciplinary collaborations that accelerate research.

Language: Английский

Citations

1

Assemblies, synapse clustering and network topology interact with plasticity to explain structure-function relationships of the cortical connectome DOI Creative Commons
András Ecker, Daniela Egas Santander, Marwan Abdellah

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 7, 2023

Synaptic plasticity underlies the brain's ability to learn and adapt. While experiments in brain slices have revealed mechanisms protocols for induction of between pairs neurons, how these synaptic changes are coordinated biological neuronal networks ensure emergence learning remains poorly understood. Simulation modeling emerged as important tools study plastic networks, but yet achieve a scale that incorporates realistic network structure, active dendrites, multi-synapse interactions, key determinants plasticity. To rise this challenge, we endowed an existing large-scale cortical model, incorporating data-constrained dendritic processing multi-synaptic connections, with calcium-based model functional captures diversity excitatory connections extrapolated vivo-like conditions. This allowed us dendrites structure interact shape stimulus representations at microcircuit level. In our exploratory simulations, acted sparsely specifically, firing rates weight distributions remained stable without additional homeostatic mechanisms. At circuit level, found was driven by co-firing stimulus-evoked assemblies, spatial clustering synapses on topology connectivity. As result changes, became more reliable stimulus-specific responses. We confirmed testable predictions MICrONS datasets, openly available electron microscopic reconstruction large volume tissue. Our results quantify architecture higher-order microcircuits play central role provide foundation elucidating their learning.

Language: Английский

Citations

3

An extended and improved CCFv3 annotation and Nissl atlas of the entire mouse brain DOI Creative Commons
Sébastien Piluso, Csaba Verasztó, Harry Carey

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 8, 2024

ABSTRACT Brain atlases are essential for quantifying cellular composition in mouse brain regions. The Allen Institute’s Common Coordinate Framework version 3 (CCFv3) is widely used, delineating over 600 anatomical regions, but it lacks coverage the most rostral and caudal parts, including main olfactory bulb, cerebellum, medulla. Additionally, CCFv3 omits key cerebellar layers, its corresponding Nissl-stained reference volume not precisely aligned, limiting utilisability. To address these issues, we developed an extended atlas, Blue Project augmented (CCFv3aBBP), which includes a fully annotated improved Nissl aligned CCFv3. This enhanced atlas also features central nervous system annotation (CCFv3cBBP). Using this resource, 734 brains to produce average template, enabling updated distribution of neuronal soma positions. These data available as open-source broadening applications such alignment precision, cell type mapping, multimodal integration.

Language: Английский

Citations

0