Synaptic connectome of theDrosophilacircadian clock

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 13, 2023

The circadian clock and its output pathways play a pivotal role in optimizing daily processes. To obtain novel insights into how diverse rhythmic physiology behaviors are orchestrated, we have generated the first comprehensive connectivity map of an animal using Drosophila FlyWire brain connectome. Intriguingly, identified additional dorsal neurons, thus showing that network contains ∼240 instead 150 neurons. We also revealed extensive contralateral synaptic within discovered indirect light input to Interestingly, observed sparse monosynaptic between neurons down-stream higher-order centers neurosecretory cells known regulate behavior physiology. Therefore, integrated single-cell transcriptomics receptor mapping decipher putative paracrine peptidergic signaling by Our analyses neuropeptides expressed suggest significantly enriches interconnectivity network.

Language: Английский

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Synaptic connectome of the Drosophila circadian clock

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 5, 2024

Abstract The circadian clock and its output pathways play a pivotal role in optimizing daily processes. To obtain insights into how diverse rhythmic physiology behaviors are orchestrated, we have generated comprehensive connectivity map of an animal using the Drosophila FlyWire brain connectome. Intriguingly, identified additional dorsal neurons, thus showing that network contains ~240 instead 150 neurons. We revealed extensive contralateral synaptic within discovered novel indirect light input to also elucidated via which modulates descending neurons known regulate feeding reproductive behaviors. Interestingly, observed sparse monosynaptic between downstream higher-order centers neurosecretory cells behavior physiology. Therefore, integrated single-cell transcriptomics receptor mapping decipher putative paracrine peptidergic signaling by Our analyses neuropeptides expressed suggest significantly enriches interconnectivity network.

Language: Английский

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Synaptic connectome of a neurosecretory network in the Drosophila brain

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 29, 2024

Abstract Hormones mediate inter-organ signaling which is crucial in orchestrating diverse behaviors and physiological processes including sleep activity, feeding, growth, metabolism reproduction. The pars intercerebralis lateralis insects represent major hubs contain neurosecretory cells (NSC) that produce various hormones. To obtain insight into how hormonal regulated, we have characterized the synaptic connectome of NSC adult Drosophila brain. Identification neurons providing inputs to multiple subtypes implicates diuretic hormone 44-expressing as a coordinator physiology behavior. Surprisingly, despite most having dendrites subesophageal zone (primary taste processing center), gustatory are largely indirect. We also deciphered pathways via olfactory relayed NSC. Further, our analyses revealed substantial from descending NSC, suggesting regulate both endocrine motor output synchronize changes with appropriate behaviors. In contrast inputs, sparse mostly mediated by corazonin Therefore, additionally determine putative paracrine interconnectivity between peripheral tissues analyzing single-cell transcriptomic datasets. Our comprehensive characterization network provides platform understand complex networks they orchestrate animal physiology.

Language: Английский

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Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila

Published: July 2, 2024

Insulin plays a key role in regulating metabolic homeostasis across vertebrate and invertebrate species. Drosophila Insulin-Producing Cells (IPCs) are functional analogues to mammalian pancreatic beta cells release insulin directly into circulation. IPC activity is modulated by nutrient availability, circadian time, the behavioral state of animals. To investigate vivo dynamics context homeostasis, we quantified effects nutritional internal changes on IPCs using electrophysiological recordings. We found that strongly modulates activity. were less active starved flies than fed flies. Refeeding with glucose significantly increased activity, suggesting regulated hemolymph sugar levels. In contrast feeding, perfusion had no effect This was reminiscent incretin effect, which ingestion drives higher intravenous application. Contrary IPCs, Diuretic hormone 44-expressing neurons pars intercerebralis (DH44 PI Ns), anatomically similar their during perfusion. Functional connectivity experiments based optogenetic activation demonstrated glucose-sensing DH44 Ns do not affect while other DH44Ns inhibit IPCs. suggests populations autonomously systemically working parallel maintain homeostasis. Ultimately, animal behavior. For example, hungry increase locomotor search food support this idea, activating small, satiety-like flies, resulting reduced walking whereas Taken together, show an integral part sophisticated modulatory network orchestrates adaptive behavior response shifts state.

Language: Английский

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eLife assessment: Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila

Published: July 2, 2024

Insulin plays a key role in regulating metabolic homeostasis across vertebrate and invertebrate species. Drosophila Insulin-Producing Cells (IPCs) are functional analogues to mammalian pancreatic beta cells release insulin directly into circulation. IPC activity is modulated by nutrient availability, circadian time, the behavioral state of animals. To investigate vivo dynamics context homeostasis, we quantified effects nutritional internal changes on IPCs using electrophysiological recordings. We found that strongly modulates activity. were less active starved flies than fed flies. Refeeding with glucose significantly increased activity, suggesting regulated hemolymph sugar levels. In contrast feeding, perfusion had no effect This was reminiscent incretin effect, which ingestion drives higher intravenous application. Contrary IPCs, Diuretic hormone 44-expressing neurons pars intercerebralis (DH44PINs), anatomically similar their during perfusion. Functional connectivity experiments based optogenetic activation demonstrated glucose-sensing DH44PINs do not affect while other DH44Ns inhibit IPCs. suggests populations autonomously systemically working parallel maintain homeostasis. Ultimately, animal behavior. For example, hungry increase locomotor search food support this idea, activating small, satiety-like flies, resulting reduced walking whereas Taken together, show an integral part sophisticated modulatory network orchestrates adaptive behavior response shifts state.

Language: Английский

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Reviewer #3 (Public Review): Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila

Published: July 2, 2024

Insulin plays a key role in regulating metabolic homeostasis across vertebrate and invertebrate species. Drosophila Insulin-Producing Cells (IPCs) are functional analogues to mammalian pancreatic beta cells release insulin directly into circulation. IPC activity is modulated by nutrient availability, circadian time, the behavioral state of animals. To investigate vivo dynamics context homeostasis, we quantified effects nutritional internal changes on IPCs using electrophysiological recordings. We found that strongly modulates activity. were less active starved flies than fed flies. Refeeding with glucose significantly increased activity, suggesting regulated hemolymph sugar levels. In contrast feeding, perfusion had no effect This was reminiscent incretin effect, which ingestion drives higher intravenous application. Contrary IPCs, Diuretic hormone 44-expressing neurons pars intercerebralis (DH44PINs), anatomically similar their during perfusion. Functional connectivity experiments based optogenetic activation demonstrated glucose-sensing DH44PINs do not affect while other DH44Ns inhibit IPCs. suggests populations autonomously systemically working parallel maintain homeostasis. Ultimately, animal behavior. For example, hungry increase locomotor search food support this idea, activating small, satiety-like flies, resulting reduced walking whereas Taken together, show an integral part sophisticated modulatory network orchestrates adaptive behavior response shifts state.

Language: Английский

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Reviewer #1 (Public Review): Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila

Published: July 2, 2024

Insulin plays a key role in regulating metabolic homeostasis across vertebrate and invertebrate species. Drosophila Insulin-Producing Cells (IPCs) are functional analogues to mammalian pancreatic beta cells release insulin directly into circulation. IPC activity is modulated by nutrient availability, circadian time, the behavioral state of animals. To investigate vivo dynamics context homeostasis, we quantified effects nutritional internal changes on IPCs using electrophysiological recordings. We found that strongly modulates activity. were less active starved flies than fed flies. Refeeding with glucose significantly increased activity, suggesting regulated hemolymph sugar levels. In contrast feeding, perfusion had no effect This was reminiscent incretin effect, which ingestion drives higher intravenous application. Contrary IPCs, Diuretic hormone 44-expressing neurons pars intercerebralis (DH44PINs), anatomically similar their during perfusion. Functional connectivity experiments based optogenetic activation demonstrated glucose-sensing DH44PINs do not affect while other DH44Ns inhibit IPCs. suggests populations autonomously systemically working parallel maintain homeostasis. Ultimately, animal behavior. For example, hungry increase locomotor search food support this idea, activating small, satiety-like flies, resulting reduced walking whereas Taken together, show an integral part sophisticated modulatory network orchestrates adaptive behavior response shifts state.

Language: Английский

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Reviewer #2 (Public Review): Nutritional state-dependent modulation of Insulin-Producing Cells in Drosophila

Published: July 2, 2024

Insulin plays a key role in regulating metabolic homeostasis across vertebrate and invertebrate species. Drosophila Insulin-Producing Cells (IPCs) are functional analogues to mammalian pancreatic beta cells release insulin directly into circulation. IPC activity is modulated by nutrient availability, circadian time, the behavioral state of animals. To investigate vivo dynamics context homeostasis, we quantified effects nutritional internal changes on IPCs using electrophysiological recordings. We found that strongly modulates activity. were less active starved flies than fed flies. Refeeding with glucose significantly increased activity, suggesting regulated hemolymph sugar levels. In contrast feeding, perfusion had no effect This was reminiscent incretin effect, which ingestion drives higher intravenous application. Contrary IPCs, Diuretic hormone 44-expressing neurons pars intercerebralis (DH44PINs), anatomically similar their during perfusion. Functional connectivity experiments based optogenetic activation demonstrated glucose-sensing DH44PINs do not affect while other DH44Ns inhibit IPCs. suggests populations autonomously systemically working parallel maintain homeostasis. Ultimately, animal behavior. For example, hungry increase locomotor search food support this idea, activating small, satiety-like flies, resulting reduced walking whereas Taken together, show an integral part sophisticated modulatory network orchestrates adaptive behavior response shifts state.

Language: Английский

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