TaNF-Y-PRC2 orchestrates temporal control of starch and protein synthesis in wheat DOI Open Access
Jinchao Chen, Long Zhao, Haoran Li

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 26, 2023

Abstract The endosperm in cereal grains is instrumental determining grain yield and seed quality, as it controls the production of starch protein. In this study, we identified a specific TaNF-Y trimeric complex, consisting TaNF-YA3-D, TaNF-YB7-B, TaNF-YC6-B, exhibiting robust expression within during filling stage wheat. Knock-down either TaNF-YA3 or TaNF-YC6 led to less but more gluten proteins. Detailed analyses have unveiled that indirectly boosts biosynthesis genes by reducing TaNAC019, repressor TaAGPS1a, TaSuS2 , thereby regulating biosynthesis. Conversely, directly inhibits gliadin low molecular weight (LMW)-GS coding genes, including TaGli-γ-700 TaLMW-400 . Furthermore, components interact with TaSWN, histone methyltransferase subunit Polycomb repressive complex 2 (PRC2), repress TaNAC019 through H3K27me3 modification. Notably, weak mutation TaFIE core PRC2, has reduced elevated LMW-GS levels. Intriguingly, DNA variations are widely associated developmental traits. particular, variation region TaNF-YB7-B linked differences protein content. Distinct haplotypes affect its interaction influencing repression targets like Our findings illuminate intricate mechanisms governing epigenetic regulation TaNF-Y-PRC2 for wheat development. Manipulating holds potential optimizing enhancing quality.

Language: Английский

The wheat powdery mildew resistance gene Pm4 also confers resistance to wheat blast DOI Creative Commons
Tom O’Hara, Andrew Steed, Rachel Goddard

et al.

Nature Plants, Journal Year: 2024, Volume and Issue: 10(6), P. 984 - 993

Published: June 19, 2024

Abstract Wheat blast, caused by the fungus Magnaporthe oryzae , threatens global cereal production since its emergence in Brazil 1985 and recently spread to Bangladesh Zambia. Here we demonstrate that AVR-Rmg8 effector, common wheat-infecting isolates, is recognized gene Pm4 previously shown confer resistance specific races of Blumeria graminis f. sp. tritici cause powdery mildew wheat. We show alleles differ their recognition different alleles, some only seedling leaves but not spikes, making it important select for those function both tissues. This study has identified a recognizing an virulence factor present wheat blast isolates Zambia represents first step towards developing durably resistant cultivars these regions.

Language: Английский

Citations

10
Development of a next generation SNP genotyping array for wheat DOI Creative Commons
Amanda Burridge, Mark Winfield, Alexandra M. Przewieslik‐Allen

et al.

Plant Biotechnology Journal, Journal Year: 2024, Volume and Issue: 22(8), P. 2235 - 2247

Published: March 23, 2024

Summary High‐throughput genotyping arrays have provided a cost‐effective, reliable and interoperable system for hexaploid wheat its relatives. Existing, highly cited including our 35K Wheat Breeder's array the Illumina 90K were designed based on limited amount of varietal sequence diversity with imperfect knowledge SNP positions. Recent progress in sequencing has given us access to vast pool diversity, whilst technological improvements allowed fit significantly more probes onto 384‐well format Axiom than previously possible. Here we describe novel array, ‘ Triticum aestivum Next Generation’ (TaNG), largely derived from whole genome skim 204 elite lines 111 landraces taken Watkins ‘Core Collection’. We used haplotype optimization approach select SNPs highest combined discrimination design iteration step test replace which failed convert markers. The final 43 372 contains combination haplotype‐optimized legacy cross‐platform show that this an improved distribution compared previous can be generate genetic maps higher number distinct bins array. also demonstrate performance TaNGv1.1 Genome‐wide association studies (GWAS) utility Copy Number Variation (CNV) analysis. is commercially available supporting marker annotations initial results freely available.

Language: Английский

Citations

5
Wheat genetic resources have avoided disease pandemics, improved food security, and reduced environmental footprints: A review of historical impacts and future opportunities DOI Creative Commons
Julie King, Susanne Dreisigacker, Matthew Reynolds

et al.

Global Change Biology, Journal Year: 2024, Volume and Issue: 30(8)

Published: Aug. 1, 2024

The use of plant genetic resources (PGR)-wild relatives, landraces, and isolated breeding gene pools-has had substantial impacts on wheat for resistance to biotic abiotic stresses, while increasing nutritional value, end-use quality, grain yield. In the Global South, post-Green Revolution yield gains are generally achieved with minimal additional inputs. As a result, production has increased, millions hectares natural ecosystems have been spared. Without PGR-derived disease resistance, fungicide would easily doubled, massively selection pressure resistance. It is estimated that in wheat, billion liters application avoided just since 2000. This review presents examples successful PGR including relentless battle against rust epidemics/pandemics, defending diseases jump species barriers like blast, biofortification giving nutrient-dense varieties novel variation improving polygenic traits climate resilience. Crop genepools urgently need be diversified increase yields across range environments (>200 Mha globally), under less predictable weather stress pressure, input efficiency. Given ~0.8 m collections worldwide relatively untapped massive tiny fraction studied, larger scale screenings introgression promise solutions emerging challenges, facilitated by advanced phenomic genomic tools. first translocations modify rhizosphere microbiome interaction (reducing biological nitrification, reducing greenhouse gases, nitrogen efficiency) landmark proof concept. Phenomics next-generation sequencing already elucidated exotic haplotypes associated complex now mainstreamed breeding. Big data from decades global trials can elucidate benefits environments. kind impact cannot without widescale sharing germplasm other technologies through networks public-private partnerships pre-competitive space.

Language: Английский

Citations

5
Unraveling wheat endosperm development: epigenetic regulation and novel regulators for enhanced yield and quality DOI Open Access
Long Zhao, Jinchao Chen,

Zhaoheng Zhang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 8, 2024

Starch content and seed storage protein (SSP) composition are critical factors influencing wheat grain yield quality. To uncover the molecular mechanisms governing their biosynthesis, we conducted transcriptome epigenome profiling across key endosperm developmental stages, revealing that chromatin accessibility, H3K27ac, H3K27me3 collectively regulate SSP starch genes with varying impact. Population phenotype analyses highlighted crucial role of accessible promoter regions as a genetic variation resource, quality in core collection accessions. By integrating time-serial RNA-seq ATAC-seq data, constructed hierarchical transcriptional regulatory network (TRN) identifying 42 high-confidence novel candidates. These candidates exhibited overlap associated size traits, functional significance was validated through expression-phenotype association analysis among accessions TILLING mutants. In-depth abscisic acid insensitive 3-A1 (TaABI3-A1) genome editing knock-out lines demonstrated its promoting accumulation while repressing biosynthesis regulation. An elite haplotype TaABI3-A1 higher weight identified during breeding process China, superior trait altered expression levels. Additionally, potential upstream regulator, GAGA-binding transcription factor 1 (TaGBP1), expression. Our study provides regulators, presenting an effective strategy for understanding regulation contributing to enhancement.

Language: Английский

Citations

2
Distinct roles of H3K27me3 and H3K36me3 in vernalization response, maintenance, and resetting in winter wheat DOI
Xuemei Liu, Min Deng,

Bingxin Shi

et al.

Science China Life Sciences, Journal Year: 2024, Volume and Issue: 67(10), P. 2251 - 2266

Published: July 8, 2024

Language: Английский

Citations

2
<i>Triticeae</i> in Global Food Security: Challenges and Prospects DOI Open Access

Renxiang Cai

Triticeae Genomics and Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Against the backdrop of global population growth, intensified climate change, and resource scarcity, stable production wheat crops such as wheat, barley, rye is crucial for ensuring food security. The Triticae plays a role in This study focuses on analyzing classification, genetic diversity, agricultural significance, major challenges faced by species family (wheat, rye), revealing importance supply, providing nutritional value, promoting sustainable development. At same time, impact biotic abiotic stress factors diseases pests, drought, etc. yield was explored, well prospects improving productivity through breeding, biotechnology, innovative practices. aim to provide scientific basis formulation security strategies promote healthy development system.

Language: Английский

Citations

0
Development of a Next Generation SNP Genotyping Array for Wheat DOI Creative Commons
Amanda Burridge, Mark Winfield, Alexandra M. Przewieslik‐Allen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 27, 2023

Summary High throughput genotyping arrays have provided a cost effective, reliable and interoperable system for hexaploid wheat its related germplasm pool. Existing, highly cited including our 35K Axiom Wheat Breeder’s array the Illumina 90K iSelect were designed based on limited amount of varietal sequence diversity with imperfect knowledge SNP positions. Recent progress in sequencing varieties landraces has given us access to vast pool diversity, whilst technological improvements design allowed fit significantly more probes onto 384-well format than was previously possible. Here we describe novel Density array, Triticum aestivum Next Generation (TaNG), largely derived from whole genome skim 204 elite lines 111 taken Watkins “Core Collection”. We use “minimal marker” optimisation approach select up six SNPs each 1.5 MB region highest combined discrimination potential. A iteration step test replace skim-sequence which failed convert markers, resulting final design, designated TaNG1.1 43,372 haplotype-optimised combination SNPs, DArTAG-derived legacy markers. show that this an even distribution across chromosomes sub-genomes compared previous can be used generate genetic maps higher number distinct bins array. also demonstrate improved performance Genome Wide Association Studies (GWAS) utility Copy Number Variation (CNV) analysis. The is commercially available, marker annotations, initial results software optimised sets are freely available.

Language: Английский

Citations

0
TaNF-Y-PRC2 orchestrates temporal control of starch and protein synthesis in wheat DOI Open Access
Jinchao Chen, Long Zhao, Haoran Li

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 26, 2023

Abstract The endosperm in cereal grains is instrumental determining grain yield and seed quality, as it controls the production of starch protein. In this study, we identified a specific TaNF-Y trimeric complex, consisting TaNF-YA3-D, TaNF-YB7-B, TaNF-YC6-B, exhibiting robust expression within during filling stage wheat. Knock-down either TaNF-YA3 or TaNF-YC6 led to less but more gluten proteins. Detailed analyses have unveiled that indirectly boosts biosynthesis genes by reducing TaNAC019, repressor TaAGPS1a, TaSuS2 , thereby regulating biosynthesis. Conversely, directly inhibits gliadin low molecular weight (LMW)-GS coding genes, including TaGli-γ-700 TaLMW-400 . Furthermore, components interact with TaSWN, histone methyltransferase subunit Polycomb repressive complex 2 (PRC2), repress TaNAC019 through H3K27me3 modification. Notably, weak mutation TaFIE core PRC2, has reduced elevated LMW-GS levels. Intriguingly, DNA variations are widely associated developmental traits. particular, variation region TaNF-YB7-B linked differences protein content. Distinct haplotypes affect its interaction influencing repression targets like Our findings illuminate intricate mechanisms governing epigenetic regulation TaNF-Y-PRC2 for wheat development. Manipulating holds potential optimizing enhancing quality.

Language: Английский

Citations

0