bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: July 5, 2024
ABSTRACT Comorbidity between psychiatric traits is thought to involve overlapping pleiotropic effects from sets of genes. Notably, substance abuse a shared comorbid condition among various neurodevelopmental disorders with externalizing symptoms such as autism spectrum disorder and attention-deficit hyperactivity disorder, thus hinting at the nucleus accumbens (NAc) site for predisposition underlying convergence genetic influences in reward-related comorbidity. Here, we identify autism-related gene encoding adhesion G protein-coupled receptor (aGPCR) Latrophilin-1/ADGRL1 an essential transducer reward mechanisms NAc. We found that ADGRL1 mRNA ubiquitously expressed throughout major NAc neuronal populations mice. A mouse model pan-neuronal Adgrl1 deficiency displayed cocaine-seeking impairments adult individuals denoting its role drug-induced reinforcement reward. Connecting molecular pathways cocaine-induced learning, uncover constitutes functional cocaine effector molecule hevin/SPARCL1. Indeed, hevin interacts membrane-expressed induces internalization while stabilizing uncleaved fraction. Moreover, alters formation intercellular contacts mediated by Neurexin-1. Importantly, constitutive coupling protein selectively modulated stimulation bias toward Gi3, Gs, G13 proteins. These findings unveil dual risk factors both define etiology
Language: Английский