The complete genome of Tetragonisca angustula (Apidae: Meliponini), the most commonly reared stingless bee in Brazil DOI Creative Commons
Rafael R. Ferrari, Paulo Cseri Ricardo, Felipe Cordeiro Dias

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 8, 2024

Abstract Background The field of bee genomics has been considerably advanced in recent years, however, the most diverse group honey producers on planet, stingless bees (SLBs), are still largely neglected. In fact, only ten ~600 described SLB species have sequenced, and one using a long-read (LR) sequencing technology. Here, we sequenced complete genome common, widespread broadly reared Brazil— Tetragonisca angustula (popularly known as jataí). Results A total 48.01 Gb DNA data were generated, including 2.31 Pacific Bioscience LRs 45.70 Illumina short reads (SRs). Our preferred assembly comprised 705 contigs encompassing 283.99 Mb, 65.94 Mb which (23.22%) corresponded to 462,261 repetitive elements. N50, L50 BUSCOs reached 1.01 94 97%, respectively. We predicted that T. comprises 16,958 protein-coding genes 1,866 non-coding RNAs. mitogenome consisted 17,410 bp, all 37 found be positive strand, an unusual feature among bees. phylogenomic analysis 26 hymenopteran revealed six 64 orthogroups experiencing rapid evolution included odorant receptor genes, but undergoing considerable contractions. Conclusions provided first for ecologically economically important , second with LR technology thus far. demonstrated even relatively small amounts combination sufficient SR can yield high-quality assemblies

Language: Английский

The complete genome of Tetragonisca angustula (Apidae: Meliponini), the most commonly reared stingless bee in Brazil DOI Creative Commons
Rafael R. Ferrari, Paulo Cseri Ricardo, Felipe Cordeiro Dias

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 8, 2024

Abstract Background The field of bee genomics has been considerably advanced in recent years, however, the most diverse group honey producers on planet, stingless bees (SLBs), are still largely neglected. In fact, only ten ~600 described SLB species have sequenced, and one using a long-read (LR) sequencing technology. Here, we sequenced complete genome common, widespread broadly reared Brazil— Tetragonisca angustula (popularly known as jataí). Results A total 48.01 Gb DNA data were generated, including 2.31 Pacific Bioscience LRs 45.70 Illumina short reads (SRs). Our preferred assembly comprised 705 contigs encompassing 283.99 Mb, 65.94 Mb which (23.22%) corresponded to 462,261 repetitive elements. N50, L50 BUSCOs reached 1.01 94 97%, respectively. We predicted that T. comprises 16,958 protein-coding genes 1,866 non-coding RNAs. mitogenome consisted 17,410 bp, all 37 found be positive strand, an unusual feature among bees. phylogenomic analysis 26 hymenopteran revealed six 64 orthogroups experiencing rapid evolution included odorant receptor genes, but undergoing considerable contractions. Conclusions provided first for ecologically economically important , second with LR technology thus far. demonstrated even relatively small amounts combination sufficient SR can yield high-quality assemblies

Language: Английский

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