Allele Sails: launching traits and fates into wild populations with DNA sequence modifiers DOI Creative Commons
Michelle L. Johnson, Bruce A. Hay, Maciej Maselko

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 19, 2024

Population-scale genome editing can be used to alter the composition or fate of wild populations. One approach achieving these aims utilizes a synthetic gene drive element-a multi-gene cassette-to bring about an increase in frequency existing allele. However, use drives is complicated by multiple scientific, regulatory, and social issues associated with transgene persistence flow. Alternatives which transgenes are not driven could potentially avoid some issues. Here we propose population scale using system refer as Allele Sail. An Sail consists editor (the Wind) that introduces DNA sequence edits Sail) at one more sites, resulting progeny viable fertile. The editor, such sequence-specific nuclease, prime- base-editor, inherited Mendelian fashion. Meanwhile, it creates experience arithmetic, Super-Mendelian frequency. We explore this agent-based modeling, identify contexts single, low release brings very high also conditions manipulation sex determination suppression. Current regulatory frameworks often distinguish between transgenics genetically modified organisms (GMOs), their edited non-transgenic non-GMO. In context provides path traits fates populations ways may considered acceptable.

Language: Английский

Identification of novel genes responsible for a pollen killer present in local natural populations of Arabidopsis thaliana DOI Creative Commons

Anthony Ricou,

Matthieu Simon,

Rémi Duflos

et al.

PLoS Genetics, Journal Year: 2025, Volume and Issue: 21(1), P. e1011451 - e1011451

Published: Jan. 13, 2025

Gamete killers are genetic loci that distort segregation in the progeny of hybrids because killer allele promotes elimination gametes carry sensitive allele. They widely distributed eukaryotes and important for understanding genome evolution speciation. We had previously identified a pollen between two distant natural accessions Arabidopsis thaliana . This involves three genetically linked genes, we reported identification gene encoding antidote protects grains from activity. In this study, other genes by using CRISPR-Cas9 induced mutants. These necessary activity demonstrated to be specific pollen. The cellular localization encoded proteins suggests mitochondria. Sequence analyses reveal predicted domains same families proteins. addition, C-terminal half one is identical antidote, amino acid, crucial activity, also essential function. Investigating more than 700 worldwide A , confirmed locus subject structural rearrangements copy number variation. By exploiting available de novo genomic sequences, propose scenario emergence Furthermore, report co-occurrence behavior genotypes several local populations, prerequisite studying gamete wild. highlights potential model not only functional studies but investigating their evolutionary trajectories at complementary geographical scales.

Language: Английский

Citations

1
Altering traits and fates of wild populations with Mendelian DNA sequence modifying Allele Sails DOI Creative Commons
Michelle L. Johnson, Bruce A. Hay, Maciej Maselko

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Aug. 13, 2024

Population-scale genome modification can alter the composition or fate of wild populations. Synthetic gene drives provide one set tools, but their use is complicated by scientific, regulatory, and social issues associated with transgene persistence flow. Here we propose an alternative approach. An Allele Sail consists a editor (the Wind) that introduces DNA sequence edits, inherited in Mendelian fashion. Meanwhile, edits Sail) experience arithmetic, Super-Mendelian increase frequency. We model this system identify contexts which single, low frequency release brings to very high also conditions manipulation sex determination bring about population suppression. In regulatory frameworks distinguish between transgenics (GMO) edited non-transgenic progeny (non-GMO) Sails may prove useful since spread GM component be limited. authors introduce as method for spreading genetic changes throughout population.

Language: Английский

Citations

3
Gene drive-based population suppression in the malaria vectorAnopheles stephensi DOI Creative Commons
Xuejiao Xu,

Jingheng Chen,

You Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 24, 2024

Abstract Gene drives are alleles that can bias the inheritance of specific traits in target populations for purpose modification or suppression. Here, we constructed a homing suppression drive major urban malaria vector Anopheles stephensi targeting female-specific exon doublesex , incorporating two gRNAs and nanos- Cas9 promoter to reduce functional resistance improve female heterozygote fitness. Our result showed was recessive sterile both females males, with various intersex phenotypes homozygotes. Both male heterozygotes only moderate conversion, indicating nos has lower activity A. than gambiae . By amplicon sequencing, detected very low level allele formation. Combination vasa -Cas9 line demonstrated boost conversion rate 100%, suggesting use similar systems population continuous release strategy SIT fRIDL techniques. This study contributes valuable insights development more efficient environmentally friendly pest control tools aimed at disrupting disease transmission.

Language: Английский

Citations

2
Allele Sails: launching traits and fates into wild populations with DNA sequence modifiers DOI Creative Commons
Michelle L. Johnson, Bruce A. Hay, Maciej Maselko

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 19, 2024

Population-scale genome editing can be used to alter the composition or fate of wild populations. One approach achieving these aims utilizes a synthetic gene drive element-a multi-gene cassette-to bring about an increase in frequency existing allele. However, use drives is complicated by multiple scientific, regulatory, and social issues associated with transgene persistence flow. Alternatives which transgenes are not driven could potentially avoid some issues. Here we propose population scale using system refer as Allele Sail. An Sail consists editor (the Wind) that introduces DNA sequence edits Sail) at one more sites, resulting progeny viable fertile. The editor, such sequence-specific nuclease, prime- base-editor, inherited Mendelian fashion. Meanwhile, it creates experience arithmetic, Super-Mendelian frequency. We explore this agent-based modeling, identify contexts single, low release brings very high also conditions manipulation sex determination suppression. Current regulatory frameworks often distinguish between transgenics genetically modified organisms (GMOs), their edited non-transgenic non-GMO. In context provides path traits fates populations ways may considered acceptable.

Language: Английский

Citations

0