Correlation of in vitro biofilm formation capacity with persistence of antibiotic-resistant Escherichia coli on gnotobiotic lamb’s lettuce DOI Creative Commons
R. Schlechter, Elisabet Marti, Mitja N. P. Remus‐Emsermann

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

ABSTRACT Bacterial contamination of fresh produce is a growing concern for food safety, as apart from human pathogens, antibiotic-resistant bacteria (ARB) can persist on leafy produce. A prominent persistence trait in biofilm formation, it provides increased tolerance to stressful conditions. We screened comprehensive collection 174 antibiotic-susceptible and -resistant Escherichia coli originating its production environment. tested the ability these strains biofilms, ranging none or weak extreme biofilm-forming bacteria. Next, we selected isolates colonize gnotobiotic lamb’s lettuce ( Valerianella locusta ) plants. hypothesized that higher vitro formation capacity correlates with colonization plant leaves. Despite marked difference form biofilms number E. strains, was not associated survival V. leaf surfaces. However, all persisted at least 21 days, highlighting potential safety risks through unwanted ingestion resistant Population densities exhibited complex pattern, subpopulations more successful colonizing These findings emphasize behavior ARB surfaces their implications safety. IMPORTANCE Each raw contains microorganisms, including This special importance such salads herbs, foods are usually consumed after minimal processing, whereby loads living ingested than heated before consumption. common bacterial lifestyle involves large groups embedded secreted protective substances. Such assemblies, so-called confer high resistance external harsh In our research, investigated whether stronger by better Although no clear correlation observed between population density salad, could survive 3 weeks significant decline over time, risk independently formation.

Language: Английский

Life on a leaf: the epiphyte to pathogen continuum and interplay in the phyllosphere DOI Creative Commons
Graham H. Thomas, William T. Kay, Helen N. Fones

et al.

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Aug. 7, 2024

Epiphytic microbes are those that live for some or all of their life cycle on the surface plant leaves. Leaf surfaces a topologically complex, physicochemically heterogeneous habitat is home to extensive, mixed communities resident and transient inhabitants from three domains life. In this review, we discuss origins leaf how different biotic abiotic factors shape communities. We as microbial adaptations which allow species thrive there, with particular emphasis occupy continuum between epiphytic specialists phytopathogens, groups have considerable overlap in terms adapting single virulence determinant can move strain. Finally, recent findings wheat pathogenic fungus Zymoseptoria tritici spends amount time surface, ask what insights other organisms might provide into pathogen, well Z. serve model system investigating plant-microbe-microbe interactions surface.

Language: Английский

Citations

9
Correlation of in vitro biofilm formation capacity with persistence of antibiotic-resistant Escherichia coli on gnotobiotic lamb’s lettuce DOI Creative Commons
R. Schlechter, Elisabet Marti, Mitja N. P. Remus‐Emsermann

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

ABSTRACT Bacterial contamination of fresh produce is a growing concern for food safety, as apart from human pathogens, antibiotic-resistant bacteria (ARB) can persist on leafy produce. A prominent persistence trait in biofilm formation, it provides increased tolerance to stressful conditions. We screened comprehensive collection 174 antibiotic-susceptible and -resistant Escherichia coli originating its production environment. tested the ability these strains biofilms, ranging none or weak extreme biofilm-forming bacteria. Next, we selected isolates colonize gnotobiotic lamb’s lettuce ( Valerianella locusta ) plants. hypothesized that higher vitro formation capacity correlates with colonization plant leaves. Despite marked difference form biofilms number E. strains, was not associated survival V. leaf surfaces. However, all persisted at least 21 days, highlighting potential safety risks through unwanted ingestion resistant Population densities exhibited complex pattern, subpopulations more successful colonizing These findings emphasize behavior ARB surfaces their implications safety. IMPORTANCE Each raw contains microorganisms, including This special importance such salads herbs, foods are usually consumed after minimal processing, whereby loads living ingested than heated before consumption. common bacterial lifestyle involves large groups embedded secreted protective substances. Such assemblies, so-called confer high resistance external harsh In our research, investigated whether stronger by better Although no clear correlation observed between population density salad, could survive 3 weeks significant decline over time, risk independently formation.

Language: Английский

Citations

0