Synchrotron X-ray imaging of soft biological tissues – principles, applications and future prospects DOI Creative Commons
Jonas Albers,

Angelika Svetlove,

Elizabeth Duke

et al.

Journal of Cell Science, Journal Year: 2024, Volume and Issue: 137(20)

Published: Oct. 15, 2024

Synchrotron-based tomographic phase-contrast X-ray imaging (SRµCT or SRnCT) is a versatile isotropic three-dimensional technique that can be used to study biological samples spanning from single cells human-sized specimens. SRµCT and SRnCT take advantage of the highly brilliant coherent X-rays produced by synchrotron light source. This enables fast data acquisition enhanced image contrast for soft owing exploitation phase contrast. In this Review, we provide an overview basics behind technique, discuss its applications biologists outlook on future emerging biology. We introduce latest advances in field, such as whole human organs imaged with micron resolution, using tool virtual histology resolving neuronal connections brain.

Language: Английский

Computational microscopy with coherent diffractive imaging and ptychography DOI
Jianwei Miao

Nature, Journal Year: 2025, Volume and Issue: 637(8045), P. 281 - 295

Published: Jan. 8, 2025

Language: Английский

Citations

5
Functional mapping of the molluscan brain guided by synchrotron X-ray tomography DOI Creative Commons
Michael Crossley,

Anna Simon,

Shashidhara Marathe

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(9)

Published: Feb. 27, 2025

Molluscan brains are composed of morphologically consistent and functionally interrogable neurons, offering rich opportunities for understanding how neural circuits drive behavior. Nonetheless, detailed component-level CNS maps often lacking, total neuron numbers unknown, organizational principles remain poorly defined, limiting a full systematic characterization circuit operation. Here, we establish an accessible, generalizable approach, harnessing synchrotron X-ray tomography, to rapidly determine the three-dimensional structure multimillimeter-scale Lymnaea . Focusing on feeding ganglia, generate neuron-level reconstruction, revealing key design revising cell count estimates upward threefold. Our atlas uncovers superficial but also nonsuperficial ganglionic architecture, reveals organization in normally hidden regions—ganglionic “dark sides”—and details features single-neuron morphology, together guiding targeted follow-up functional investigation based intracellular recordings. Using this identify three pivotal classes: command-like food-signaling type, central pattern generator interneuron, unique behavior-specific motoneuron, significantly advancing function classical control circuit. Combining our morphological electrophysiological data, as shared scalable resource research community. approach enables rapid construction atlases large-scale nervous systems, with relevance interrogation diverse range model organisms.

Language: Английский

Citations

0
3D virtual histology of rodent and primate cochleae with multi-scale phase-contrast X-ray tomography DOI Creative Commons
Jannis Schaeper, Christoph A. Kampshoff, Bettina Wolf

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 7, 2025

Multi-scale X-ray phase contrast tomography (XPCT) enables three-dimensional (3D), non-destructive imaging of intact small animal cochlea and apical cochlear turns. Here we report on post-mortem excised non-human primate rodent cochleae at different [Formula: see text]-CT nano-CT synchrotron instruments. We explore sample embeddings, stainings regimes. Under optimized conditions preparation, instrumentation, protocol, retrieval, high image quality detail level can be achieved in 3D reconstructions. The showcased instrumentation protocols along with the reconstucted volumes serve as benchmarks reference for multi-scale microanatomy histology. provided this work cover a wide range scales are intended augmented tools auditory research.

Language: Английский

Citations

0
Comparison of Soft X-ray Spectro-Ptychography and Scanning Transmission X-ray Microscopy DOI Creative Commons
Adam P. Hitchcock, Chunyang Zhang, Haytham Eraky

et al.

Journal of Electron Spectroscopy and Related Phenomena, Journal Year: 2024, Volume and Issue: unknown, P. 147487 - 147487

Published: Sept. 1, 2024

Language: Английский

Citations

1
Synchrotron X-ray imaging of soft biological tissues – principles, applications and future prospects DOI Creative Commons
Jonas Albers,

Angelika Svetlove,

Elizabeth Duke

et al.

Journal of Cell Science, Journal Year: 2024, Volume and Issue: 137(20)

Published: Oct. 15, 2024

Synchrotron-based tomographic phase-contrast X-ray imaging (SRµCT or SRnCT) is a versatile isotropic three-dimensional technique that can be used to study biological samples spanning from single cells human-sized specimens. SRµCT and SRnCT take advantage of the highly brilliant coherent X-rays produced by synchrotron light source. This enables fast data acquisition enhanced image contrast for soft owing exploitation phase contrast. In this Review, we provide an overview basics behind technique, discuss its applications biologists outlook on future emerging biology. We introduce latest advances in field, such as whole human organs imaged with micron resolution, using tool virtual histology resolving neuronal connections brain.

Language: Английский

Citations

0