An atlas of conserved transcription factor binding sites reveals the cell type-resolved gene regulatory landscape of flowering plants DOI Creative Commons
Leo Baumgart, Abraham Morales‐Cruz, Sharon Greenblum

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 12, 2024

Summary Transcription factors (TFs) play a central role in regulating gene expression, process fundamental to cellular function. Characterizing transcription factor binding sites (TFBSs) is essential for understanding TF functions and identifying target genes, but many predicted TFBSs lack clear biological significance. We generated an atlas of across ten flowering plants demonstrated that conserved are strongly enriched genomic epigenomic signatures functional regulatory elements. uncovered properties TFBSs, including family-specific clustering distinct promoter body locations. By integrating this with single nuclei RNA chromatin profiles, we elucidated activity underlying development stress responses annotated key cell type-specific pathways. Finally, found ortholog specificity preserved over long evolutionary times, suggesting divergence sequences, rather than TFs, primary driver evolution.

Language: Английский

An atlas of conserved transcription factor binding sites reveals the cell type-resolved gene regulatory landscape of flowering plants DOI Creative Commons
Leo Baumgart, Abraham Morales‐Cruz, Sharon Greenblum

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 12, 2024

Summary Transcription factors (TFs) play a central role in regulating gene expression, process fundamental to cellular function. Characterizing transcription factor binding sites (TFBSs) is essential for understanding TF functions and identifying target genes, but many predicted TFBSs lack clear biological significance. We generated an atlas of across ten flowering plants demonstrated that conserved are strongly enriched genomic epigenomic signatures functional regulatory elements. uncovered properties TFBSs, including family-specific clustering distinct promoter body locations. By integrating this with single nuclei RNA chromatin profiles, we elucidated activity underlying development stress responses annotated key cell type-specific pathways. Finally, found ortholog specificity preserved over long evolutionary times, suggesting divergence sequences, rather than TFs, primary driver evolution.

Language: Английский

Citations

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