Transcription factors associated with regulation of transcriptome in human thigh and calf muscles at baseline and after one week of disuse DOI Creative Commons
Anna A. Borzykh, Pavel A. Makhnovskii, Ivan I. Ponomarev

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

Abstract Disuse has a negative impact on the postural muscles of trunk and legs. Different leg demonstrate differentiated conservative response to disuse, in terms decrease muscle mass, strength, aerobic performance, changes gene expression. We aimed identify transcription factors regulating expression at baseline after disuse human m. soleus – “slow” with strong function, “mixed” vastus lateralis . Biopsies were taken from these prior 6 days strict (dry immersion). The enriched factor binding sites (and corresponding factors) individual promoter regions co-expressed genes examined using positional weight matrix approach. transcriptomic profiles disuse-induced (RNA-seq) differ significantly between muscles. In particular, specific significant was found be strongly related suppression mitochondrial energy metabolism, activation inflammatory ubiquitin-proteasome system. This is associated proinflammatory such as families IRF, STAT, other. validity approximately two-thirds predicted indirectly confirmed by analysis their function described literature. These identified appear promising candidates for future targeted studies that mechanistically investigate regulation various baseline, following or inactivity. Highlights different transcriptome differs markedly. greater than partially inflammation-induced factors.

Language: Английский

Transcription factors associated with regulation of transcriptome in human thigh and calf muscles at baseline and after one week of disuse DOI Creative Commons
Anna A. Borzykh, Pavel A. Makhnovskii, Ivan I. Ponomarev

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 15, 2024

Abstract Disuse has a negative impact on the postural muscles of trunk and legs. Different leg demonstrate differentiated conservative response to disuse, in terms decrease muscle mass, strength, aerobic performance, changes gene expression. We aimed identify transcription factors regulating expression at baseline after disuse human m. soleus – “slow” with strong function, “mixed” vastus lateralis . Biopsies were taken from these prior 6 days strict (dry immersion). The enriched factor binding sites (and corresponding factors) individual promoter regions co-expressed genes examined using positional weight matrix approach. transcriptomic profiles disuse-induced (RNA-seq) differ significantly between muscles. In particular, specific significant was found be strongly related suppression mitochondrial energy metabolism, activation inflammatory ubiquitin-proteasome system. This is associated proinflammatory such as families IRF, STAT, other. validity approximately two-thirds predicted indirectly confirmed by analysis their function described literature. These identified appear promising candidates for future targeted studies that mechanistically investigate regulation various baseline, following or inactivity. Highlights different transcriptome differs markedly. greater than partially inflammation-induced factors.

Language: Английский

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