Multi‐drug resistant Staphylococcus epidermidis from chronic wounds impair healing in human wound model DOI Creative Commons
Miroslav Dinić,

Rebecca Verpile,

Jamie L. Burgess

et al.

Wound Repair and Regeneration, Journal Year: 2024, Volume and Issue: 32(6), P. 799 - 810

Published: Oct. 22, 2024

Abstract Venous leg ulcers (VLUs) represent one of the most prevalent types chronic wounds characterised by perturbed microbiome and biofilm‐forming bacteria. As abundant skin‐commensal, Staphylococcus epidermidis is known as beneficial for host, however, some strains can form biofilms hinder wound healing. In this study, S. distribution in VLUs associated resistome were analysed ulcer tissue from patients. Virulence isolates evaluated whole genome sequencing, antimicrobial susceptibility testing, vitro biofilm binding assays, assessment capability pro‐inflammatory potential using human ex vivo model. We demonstrated that inhibit re‐epithelialization through biofilm‐dependent induction IL‐1β, IL‐8, IL‐6 which was accordance with impaired healing outcomes observed High extracellular matrix ability VLU resistance expression levels embp sdrG , responsible bacterial to fibrinogen fibrin, respectively. Finally, we showed demonstrate pathogenic features impair underscores emergence treatment‐resistant virulent lineages patients ulcers.

Language: Английский

Morphological variability of Escherichia coli colonizing human wounds: a case report DOI Creative Commons
Xuejin Wang, Hanyu Wang, Jing Zhang

et al.

BMC Infectious Diseases, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 31, 2025

Escherichia coli, known for its adaptability, can cause diverse infections. Morphological variants, such as smooth and mucoid forms, correspond to different infection capabilities antibiotic resistance profiles. This report presents the isolation of two distinct E. coli strains, a strain strain, from single patient. A 70-year-old woman with leg wound lung was found have strains: her sputum rectal swab. Whole-genome sequencing confirmed genetic similarity between strains minor SNPs linked their morphological differences. Both were resistant β-lactam quinolone antibiotics, complicating treatment. The patient recovered following treatment Piperacillin/Tazobactam regular care. case highlights coli's phenotypic plasticity within host, impacting management response. Understanding basis changes could inform more effective strategies.

Language: Английский

Citations

0
Multi‐drug resistant Staphylococcus epidermidis from chronic wounds impair healing in human wound model DOI Creative Commons
Miroslav Dinić,

Rebecca Verpile,

Jamie L. Burgess

et al.

Wound Repair and Regeneration, Journal Year: 2024, Volume and Issue: 32(6), P. 799 - 810

Published: Oct. 22, 2024

Abstract Venous leg ulcers (VLUs) represent one of the most prevalent types chronic wounds characterised by perturbed microbiome and biofilm‐forming bacteria. As abundant skin‐commensal, Staphylococcus epidermidis is known as beneficial for host, however, some strains can form biofilms hinder wound healing. In this study, S. distribution in VLUs associated resistome were analysed ulcer tissue from patients. Virulence isolates evaluated whole genome sequencing, antimicrobial susceptibility testing, vitro biofilm binding assays, assessment capability pro‐inflammatory potential using human ex vivo model. We demonstrated that inhibit re‐epithelialization through biofilm‐dependent induction IL‐1β, IL‐8, IL‐6 which was accordance with impaired healing outcomes observed High extracellular matrix ability VLU resistance expression levels embp sdrG , responsible bacterial to fibrinogen fibrin, respectively. Finally, we showed demonstrate pathogenic features impair underscores emergence treatment‐resistant virulent lineages patients ulcers.

Language: Английский

Citations

1