Gene drive-based population suppression in the malaria vectorAnopheles stephensi DOI Creative Commons
Xuejiao Xu,

Jingheng Chen,

You Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 24, 2024

Abstract Gene drives are alleles that can bias the inheritance of specific traits in target populations for purpose modification or suppression. Here, we constructed a homing suppression drive major urban malaria vector Anopheles stephensi targeting female-specific exon doublesex , incorporating two gRNAs and nanos- Cas9 promoter to reduce functional resistance improve female heterozygote fitness. Our result showed was recessive sterile both females males, with various intersex phenotypes homozygotes. Both male heterozygotes only moderate conversion, indicating nos has lower activity A. than gambiae . By amplicon sequencing, detected very low level allele formation. Combination vasa -Cas9 line demonstrated boost conversion rate 100%, suggesting use similar systems population continuous release strategy SIT fRIDL techniques. This study contributes valuable insights development more efficient environmentally friendly pest control tools aimed at disrupting disease transmission.

Language: Английский

Gene drive-based population suppression in the malaria vectorAnopheles stephensi DOI Creative Commons
Xuejiao Xu,

Jingheng Chen,

You Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 24, 2024

Abstract Gene drives are alleles that can bias the inheritance of specific traits in target populations for purpose modification or suppression. Here, we constructed a homing suppression drive major urban malaria vector Anopheles stephensi targeting female-specific exon doublesex , incorporating two gRNAs and nanos- Cas9 promoter to reduce functional resistance improve female heterozygote fitness. Our result showed was recessive sterile both females males, with various intersex phenotypes homozygotes. Both male heterozygotes only moderate conversion, indicating nos has lower activity A. than gambiae . By amplicon sequencing, detected very low level allele formation. Combination vasa -Cas9 line demonstrated boost conversion rate 100%, suggesting use similar systems population continuous release strategy SIT fRIDL techniques. This study contributes valuable insights development more efficient environmentally friendly pest control tools aimed at disrupting disease transmission.

Language: Английский

Citations

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