CD4 T cell contact drives macrophage cell cycle progression and susceptibility to lentiviral transduction DOI Creative Commons
Petra Mlčochová, Raphael Heilig, Román Fischer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 16, 2024

Abstract Macrophages are typically quiescent cells residing in G0, though tissue macrophages have been shown to proliferate locally tissues; we previously demonstrated that differentiated monocyte derived (MDM) can be stimulated re-enter G1 phase of the cell cycle from without division. Entry into correlates with an increase CDK1 expression which phosphorylates deoxynucleotide-triphosphate hydrolase SAMHD1 at position 592. not only regulates cellular dNTP levels, but is also a restriction factor for virus replication HIV-1 and DNA viruses. Here show contact autologous CD4 T leads antigen-independent macrophage progression G0-G1, accompanied by associated proteins, including CDK1, activation canonical MEK-ERK pathway. Further, blocked anti-cancer drugs targeting axis such as Palcociclib, pre-treatment EGFR antibody, providing additional evidence surface interactions driving proliferative responses. Cell uninfected renders ten-fold more susceptible transduction VSV-G pseudotyped particles.

Language: Английский

CD4 T cell contact drives macrophage cell cycle progression and susceptibility to lentiviral transduction DOI Creative Commons
Petra Mlčochová, Raphael Heilig, Román Fischer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 16, 2024

Abstract Macrophages are typically quiescent cells residing in G0, though tissue macrophages have been shown to proliferate locally tissues; we previously demonstrated that differentiated monocyte derived (MDM) can be stimulated re-enter G1 phase of the cell cycle from without division. Entry into correlates with an increase CDK1 expression which phosphorylates deoxynucleotide-triphosphate hydrolase SAMHD1 at position 592. not only regulates cellular dNTP levels, but is also a restriction factor for virus replication HIV-1 and DNA viruses. Here show contact autologous CD4 T leads antigen-independent macrophage progression G0-G1, accompanied by associated proteins, including CDK1, activation canonical MEK-ERK pathway. Further, blocked anti-cancer drugs targeting axis such as Palcociclib, pre-treatment EGFR antibody, providing additional evidence surface interactions driving proliferative responses. Cell uninfected renders ten-fold more susceptible transduction VSV-G pseudotyped particles.

Language: Английский

Citations

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