Neuropeptide Modulation Enables Biphasic Internetwork Coordination via a Dual-Network Neuron DOI Creative Commons
Barathan Gnanabharathi, Savanna-Rae H. Fahoum, Dawn M. Blitz

et al.

eNeuro, Journal Year: 2024, Volume and Issue: 11(6), P. ENEURO.0121 - 24.2024

Published: June 1, 2024

Linked rhythmic behaviors, such as respiration/locomotion or swallowing/chewing, often require coordination for proper function. Despite its prevalence, the cellular mechanisms controlling of underlying neural networks remain undetermined in most systems. We use stomatogastric nervous system crab Cancer borealis to investigate internetwork coordination, due small, well-characterized feeding-related (gastric mill [chewing, ∼0.1 Hz]; pyloric [filtering food, ∼1 Hz]). Here, we between these during Gly 1 -SIFamide neuropeptide modulatory state. activates a unique triphasic gastric rhythm which typically pyloric-only LPG neuron generates dual pyloric-plus mill-timed oscillations. Additionally, exhibits shorter cycles rhythm-timed bursts, and longer IC, IC plus LG bursts. Photoinactivation revealed that is necessary shorten cycle period, likely through rectified electrical coupling pacemaker neurons. Hyperpolarizing current injections demonstrated although bursting enables only bursts are prolong period. Surprisingly, photoinactivation also eliminated prolonged cycles, without changing firing frequency burst duration, suggesting via synaptic inhibition LPG, indirectly slows pacemakers coupling. Thus, same dual-network directly conveys excitation from endogenous funnels enable one network alternately decrease increase period related network.

Language: Английский

Neuropeptide Modulation Enables Biphasic Inter-network Coordination via a Dual-Network Neuron DOI Creative Commons
Barathan Gnanabharathi, Savanna-Rae H. Fahoum, Dawn M. Blitz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 20, 2024

Abstract Linked rhythmic behaviors, such as respiration/locomotion or swallowing/chewing often require coordination for proper function. Despite its prevalence, the cellular mechanisms controlling of underlying neural networks remain undetermined in most systems. We use stomatogastric nervous system crab Cancer borealis to investigate inter-network coordination, due small, well characterized feeding-related (gastric mill [chewing, ∼0.1 Hz]; pyloric [filtering food, ∼1 Hz]). Here, we between these during Gly 1 -SIFamide neuropeptide modulatory state. activates a unique triphasic gastric rhythm which typically pyloric-only LPG neuron generates dual pyloric- plus mill-timed oscillations. Additionally, exhibits shorter cycles rhythm-timed bursts, and longer IC, IC LG bursts. Photoinactivation revealed that is necessary shorten cycle period, likely through rectified electrical coupling pacemaker neurons. Hyperpolarizing current injections demonstrated although bursting enables only bursts are prolong period. Surprisingly, photoinactivation also eliminated prolonged cycles, without changing firing frequency burst duration, suggesting via synaptic inhibition LPG, indirectly slows pacemakers coupling. Thus, same dual-network directly conveys excitation from endogenous funnels enable one network alternately decrease increase period related network. Significance Statement Related behaviors frequently exhibit yet coordinating not determined investigated two well-characterized crustacean feeding-associated neuropeptide-elicited find fast/slow shortens fast slow, intrinsically generated pacemakers, despite rectification favoring opposite direction. another slow-network phase, chemical funneled synapse. reinforces diminishes actions, enabling distinct frequencies faster across different phases slower rhythm.

Language: Английский

Citations

2
Neuropeptide Modulation Enables Biphasic Internetwork Coordination via a Dual-Network Neuron DOI Creative Commons
Barathan Gnanabharathi, Savanna-Rae H. Fahoum, Dawn M. Blitz

et al.

eNeuro, Journal Year: 2024, Volume and Issue: 11(6), P. ENEURO.0121 - 24.2024

Published: June 1, 2024

Linked rhythmic behaviors, such as respiration/locomotion or swallowing/chewing, often require coordination for proper function. Despite its prevalence, the cellular mechanisms controlling of underlying neural networks remain undetermined in most systems. We use stomatogastric nervous system crab Cancer borealis to investigate internetwork coordination, due small, well-characterized feeding-related (gastric mill [chewing, ∼0.1 Hz]; pyloric [filtering food, ∼1 Hz]). Here, we between these during Gly 1 -SIFamide neuropeptide modulatory state. activates a unique triphasic gastric rhythm which typically pyloric-only LPG neuron generates dual pyloric-plus mill-timed oscillations. Additionally, exhibits shorter cycles rhythm-timed bursts, and longer IC, IC plus LG bursts. Photoinactivation revealed that is necessary shorten cycle period, likely through rectified electrical coupling pacemaker neurons. Hyperpolarizing current injections demonstrated although bursting enables only bursts are prolong period. Surprisingly, photoinactivation also eliminated prolonged cycles, without changing firing frequency burst duration, suggesting via synaptic inhibition LPG, indirectly slows pacemakers coupling. Thus, same dual-network directly conveys excitation from endogenous funnels enable one network alternately decrease increase period related network.

Language: Английский

Citations

0