Genetics, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 7, 2024
Abstract Domesticated strains of Saccharomyces cerevisiae have adapted to resist copper and sulfite, 2 chemical stressors commonly used in winemaking. S. paradoxus has not these chemicals despite being consistently present sympatry with vineyards. This contrast could be driven by a number factors including niche differences or differential access resistance mutations between species. In this study, we comparative mutagenesis approach test whether is mutationally constrained respect acquiring greater sulfite resistance. For both species, assayed the rate, effect size, pleiotropic costs sequenced subset 150 mutants. We found that distributions mutational effects displayed species were similar poorly explained natural pattern. also chromosome VIII aneuploidy loss function PMA1 confer whereas REG1 was only viable route cerevisiae. observed de novo duplication CUP1 gene but RTS1 KSP1 larger paradoxus. Our results show even when available are largely similar, can differ adaptive paths them. They demonstrate assays distribution may lack predictive insight concerning outcomes.
Language: Английский