Isolation and characterization of the new Streptomyces phages Kamino, Geonosis, Abafar, and Scarif infecting a broad range of host species DOI Creative Commons
Bente Rackow, Clara Rolland,

Isabelle Mohnen

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 25, 2024

ABSTRACT Streptomyces , a multifaceted genus of soil-dwelling bacteria belonging to the phylum Actinomycetota features intricate phage-host interactions shaped by its complex life cycle and synthesis diverse array specialized metabolites. Here, we describe isolation characterization four novel phages infecting variety different host species. While phage Kamino, isolated on kasugaensis is predicted be temperate encodes serine integrase in genome, Geonosis (isolated griseus ) Abafar Scarif, albidoflavus are virulent phages. Phages Kamino were shown amplify well liquid culture leading pronounced collapse already at low titers. Determination range testing >40 species identified Abafar, Scarif as broad host-range Overall, described this study expand publicly available portfolio will instrumental advancing mechanistic understanding antiviral strategies employed these multicellular bacteria. IMPORTANCE The actinobacterial characterized multicellular, filamentous growth bioactive molecules. These characteristics also play role shaping their with most abundant predator environment, bacteriophages—viruses In study, characterize new . Out those, three feature up 15 respect plaque virion morphology, range, amplification culture. summary, reported contribute broader collection playing crucial our

Language: Английский

Isolation and characterization of the new Streptomyces phages Kamino, Geonosis, Abafar, and Scarif infecting a broad range of host species DOI Creative Commons
Bente Rackow, Clara Rolland,

Isabelle Mohnen

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 25, 2024

ABSTRACT Streptomyces , a multifaceted genus of soil-dwelling bacteria belonging to the phylum Actinomycetota features intricate phage-host interactions shaped by its complex life cycle and synthesis diverse array specialized metabolites. Here, we describe isolation characterization four novel phages infecting variety different host species. While phage Kamino, isolated on kasugaensis is predicted be temperate encodes serine integrase in genome, Geonosis (isolated griseus ) Abafar Scarif, albidoflavus are virulent phages. Phages Kamino were shown amplify well liquid culture leading pronounced collapse already at low titers. Determination range testing >40 species identified Abafar, Scarif as broad host-range Overall, described this study expand publicly available portfolio will instrumental advancing mechanistic understanding antiviral strategies employed these multicellular bacteria. IMPORTANCE The actinobacterial characterized multicellular, filamentous growth bioactive molecules. These characteristics also play role shaping their with most abundant predator environment, bacteriophages—viruses In study, characterize new . Out those, three feature up 15 respect plaque virion morphology, range, amplification culture. summary, reported contribute broader collection playing crucial our

Language: Английский

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