Chromosomes X et Y : acteurs clés des inégalités de sexe face aux maladies DOI
Agnese Loda, James Cleland, Édith Heard

et al.

Pour la Science, Journal Year: 2024, Volume and Issue: n° 567(1), P. 22 - 31

Published: Dec. 20, 2024

Single-cell RNA-seq identifies protracted mouse germline X chromosome reactivation dynamics directed by a PRC2-dependent mechanism DOI Creative Commons
Yaqiong Liu, Xianzhong Lau, Prabhakaran Munusamy

et al.

Developmental Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Female primordial germ cells (PGCs) undergo X chromosome reactivation (XCR) during genome-wide reprogramming. XCR kinetics and dynamics are poorly understood at a molecular level. Here, we apply single-cell RNA sequencing chromatin profiling on from F

Language: Английский

Citations

1
Chromosomal and gonadal sex have differing effects on social motivation in mice DOI Creative Commons
Sneha Chaturvedi, Simona Sarafinovska, Din Selmanovic

et al.

Biology of Sex Differences, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 19, 2025

Language: Английский

Citations

1
Altered X-chromosome inactivation of the TLR7/8 locus and heterogeneity of pDCs in systemic sclerosis DOI
Yong Du, Bérénice Faz-Lopez, Marie Dominique Ah Kioon

et al.

The Journal of Experimental Medicine, Journal Year: 2024, Volume and Issue: 222(3)

Published: Dec. 13, 2024

Systemic sclerosis (SSc) is an autoimmune disease that has a strong female predominance. Both the X-linked TLR7 and TLR8 can induce type I IFN (IFN-I) by plasmacytoid DCs (pDCs), which promote fibrosis. We identified five subclusters of pDCs, including ISGhigh clusters were over-represented in SSc patients. observed both genes escape from X chromosome inactivation (XCI) at higher frequency pDCs patients, was associated with changes protein profile. Combined DNA/RNA FISH analysis revealed TLR7/8 locus preferentially located outside inactive (Xi) territory when expressed, suggesting higher-order loop formation linked to expression Xi. Furthermore, levels XIST transcriptional repressor SPEN reduced pDCs. Hence, our data heterogeneity suggested altered XCI may contribute chronic IFN-I activity

Language: Английский

Citations

6
Régulation du chromosome X et spécificités fonctionnelles des cellules femelles DOI Creative Commons
Julie Chaumeil,

Céline Morey

médecine/sciences, Journal Year: 2024, Volume and Issue: 40(12), P. 935 - 946

Published: Dec. 1, 2024

Et si posséder deux chromosomes X conférait aux cellules femelles des spécificités exclusives et expliquait les différences de susceptibilité hommes femmes à certaines maladies ? Pourtant, l’un est réprimé aléatoirement dans chaque cellule femelle dès le stade embryonnaire, ce qui, théoriquement, remet sexes égalité. Cette inactivation du chromosome un processus épigénétique unique : elle agit l’échelle d’un entier aboutit mosaïcisme d’expression gènes au niveau l’organisme. Cependant certains échappent cette semble être plus labile types cellulaires. Quelles en sont conséquences physiologiques question commence explorée, notamment systèmes immunitaires nerveux qui présentent différentes biais sexe.

Citations

1
Unlocking the potential of SY-stem cells DOI
Federica Furlanetto, Sarah Frank, Marisa Karow

et al.

Development, Journal Year: 2024, Volume and Issue: 151(12)

Published: June 15, 2024

The sixth SY-Stem Symposium, jointly organized by the Research Institute of Molecular Pathology and Biotechnology took place in Vienna March 2024. Again, aspiring new group leaders were given a stage to present their work vision labs. To round up excellent program, scientific organizers included renowned keynote speakers. Here, we provide summary talks covering topics such as early embryogenesis, nervous system development disease, regeneration latest technologies.

Language: Английский

Citations

0
Reporter CRISPR screens decipher cis- and trans-regulatory principles at the Xist locus DOI Creative Commons
Till Schwämmle, Gemma Noviello,

Eleni Kanata

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 10, 2024

Abstract Developmental genes are controlled by an ensemble of cis -acting regulatory elements (REs), which in turn respond to multiple trans transcription factors (TFs). Understanding how a -regulatory landscape integrates information from many dynamically expressed TFs has remained challenge. We develop combined CRISPR-screening approach using endogenous RNA and RE-reporters as readouts. Applied the Xist locus, crucial for X-chromosome inactivation females, this method allows us comprehensively identify Xist-controlling map their TF-RE wiring. find group transiently that regulate proximal REs, driving binary activation expression. These basal activators more highly cells with two X chromosomes, potentially female-specific upregulation. A second set developmental is upregulated later during differentiation targets distal REs. This axis achieve high levels RNA, necessary inactivation. Our findings support model gene regulation targeting REs drive ON-OFF decisions, while interacting control output.

Language: Английский

Citations

0
Unleashing XIST from X-chromosome inactivation DOI Creative Commons

Céline Morey,

Claire Rougeulle, Jean-François Ouimette

et al.

Current Opinion in Cell Biology, Journal Year: 2024, Volume and Issue: 92, P. 102446 - 102446

Published: Nov. 27, 2024

Language: Английский

Citations

0
Chromosomes X et Y : acteurs clés des inégalités de sexe face aux maladies DOI
Agnese Loda, James Cleland, Édith Heard

et al.

Pour la Science, Journal Year: 2024, Volume and Issue: n° 567(1), P. 22 - 31

Published: Dec. 20, 2024

Citations

0