Cultured Bacteria Isolated from Primary Sclerosing Cholangitis Patient Bile Induce Inflammation and Cell Death DOI Open Access
Chelsea E. Powell, Megan D. McCurry,

Silvia F. Quevedo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

ABSTRACT Background Primary sclerosing cholangitis (PSC) is a chronic liver disease characterized by inflammation and progressive fibrosis of the biliary tree. The pathogenesis PSC remains poorly understood, there are no effective therapeutic options. Previous studies have observed associations between changes in colonic microbiome PSC. We aimed to determine whether bacterial isolates cultured from patient bile induced disease-associated phenotypes cells. Methods Bile was collected patients (n=10) endoscopic retrograde cholangiography non-PSC controls (n=3) undergoing cholecystectomies. Biliary bacteria were anaerobically, 50 colonies per sample identified 16S rRNA sequencing. effects supernatants seven PSC-associated strains on cellular using human (Caco-2), hepatic (HepG2), (EGI-1) Results No isolated controls, while most patients. microbiomes exhibited reduced diversity compared gut or oral cavity, with one two predominating. Overall, produced factors that cytotoxic Enterococcus faecalis , lesser extent Veillonella parvula epithelial permeability, Escherichia coli, Fusobacterium necrophorum Klebsiella pneumoniae inflammatory cytokines Conclusions Our data suggest induce may contribute pathogenesis. promote intestinal facilitating migration Once there, Escherichia, cause damage

Language: Английский

Cultured Bacteria Isolated from Primary Sclerosing Cholangitis Patient Bile Induce Inflammation and Cell Death DOI Open Access
Chelsea E. Powell, Megan D. McCurry,

Silvia F. Quevedo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 9, 2024

ABSTRACT Background Primary sclerosing cholangitis (PSC) is a chronic liver disease characterized by inflammation and progressive fibrosis of the biliary tree. The pathogenesis PSC remains poorly understood, there are no effective therapeutic options. Previous studies have observed associations between changes in colonic microbiome PSC. We aimed to determine whether bacterial isolates cultured from patient bile induced disease-associated phenotypes cells. Methods Bile was collected patients (n=10) endoscopic retrograde cholangiography non-PSC controls (n=3) undergoing cholecystectomies. Biliary bacteria were anaerobically, 50 colonies per sample identified 16S rRNA sequencing. effects supernatants seven PSC-associated strains on cellular using human (Caco-2), hepatic (HepG2), (EGI-1) Results No isolated controls, while most patients. microbiomes exhibited reduced diversity compared gut or oral cavity, with one two predominating. Overall, produced factors that cytotoxic Enterococcus faecalis , lesser extent Veillonella parvula epithelial permeability, Escherichia coli, Fusobacterium necrophorum Klebsiella pneumoniae inflammatory cytokines Conclusions Our data suggest induce may contribute pathogenesis. promote intestinal facilitating migration Once there, Escherichia, cause damage

Language: Английский

Citations

0