The PIN2 ortholog in barley modifies root gravitropism and architecture without impacting the shoot DOI Creative Commons
Zachary Aldiss, Hannah Robinson, Yasmine Lam

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 29, 2024

SUMMARY Roots provide the critical interface where plants acquire nutrients and water, but our limited understanding of genetic controls modulating root system architecture (RSA) in crop species constrains opportunities to develop future cultivars with improved systems. However, there is vast knowledge developmental genes model plant species, which has potential accelerate progress crops more complex genomes, particularly given that genome editing protocols are now available for most species. PIN-FORMED2 ( PIN2 ) encodes a specific polar auxin transporter, its absence resulted roots being unable orient themselves using gravity, producing significantly wider system. To explore role cereal crop, we used CRISPR/Cas9 knockout barley Hordeum vulgare ). Like Arabidopsis, pin2 loss-of-function mutants displayed an agravitropic response at seedling growth stages, resulting shallower later stages. Notably, despite significant change RSA, was no shoot or total biomass. We discuss challenges harness pathway optimise RSA range production scenarios without trade-off.

Language: Английский

The PIN2 ortholog in barley modifies root gravitropism and architecture without impacting the shoot DOI Creative Commons
Zachary Aldiss, Hannah Robinson, Yasmine Lam

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 29, 2024

SUMMARY Roots provide the critical interface where plants acquire nutrients and water, but our limited understanding of genetic controls modulating root system architecture (RSA) in crop species constrains opportunities to develop future cultivars with improved systems. However, there is vast knowledge developmental genes model plant species, which has potential accelerate progress crops more complex genomes, particularly given that genome editing protocols are now available for most species. PIN-FORMED2 ( PIN2 ) encodes a specific polar auxin transporter, its absence resulted roots being unable orient themselves using gravity, producing significantly wider system. To explore role cereal crop, we used CRISPR/Cas9 knockout barley Hordeum vulgare ). Like Arabidopsis, pin2 loss-of-function mutants displayed an agravitropic response at seedling growth stages, resulting shallower later stages. Notably, despite significant change RSA, was no shoot or total biomass. We discuss challenges harness pathway optimise RSA range production scenarios without trade-off.

Language: Английский

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