The defensome of Acinetobacter baumannii reveals two genome groups according to their innate defense systems and phage profile DOI Creative Commons
Antonio Moreno-Rodríguez, Alejandro Rubio, Andrés Garzón

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 27, 2024

Abstract Phages are guilty of killing daily almost half bacterial cells, while bacteria have developed defense mechanisms that number in the dozens. Individual systems gained and lost by genomes same species, depending on their fitness advantage. Thus, some a certain combination systems, other act as reservoir for rest thus constituting so-called pan-immune system species. Here we analyzed thousands bacterium Acinetobacter baumannii , an opportunistic pathogen humans great clinical concern, found 81 different heterogeneously distributed. By analyzing how these combine, more than lack universal DNA-methylating restriction-modification (R-M) harbor alternative innate SspBCDE performs DNA phosphorothioate modification. In addition, adaptive CRISPR-Cas could synergistically to R-M based frequency co-appearance. The presence one or modulate evolution this causing them present profile phages integrated into genome. We also observed many is associated with higher prophages, which be due fact prophage carries system, would not need environments where phage absent.

Language: Английский

Evolution of a vascular plant pathogen is associated with the loss of CRISPR-Cas and an increase in genome plasticity and virulence genes DOI
Misha Paauw,

Willem E.W. Schravesande,

Nanne W. Taks

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

1
Pipolins are bimodular platforms that maintain a reservoir of defense systems exchangeable with various bacterial genetic mobile elements DOI Creative Commons

Víctor Mateo-Cáceres,

Modesto Redrejo‐Rodríguez

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

Abstract Defense genes gather in diverse types of genomic islands bacteria and provide immunity against viruses other genetic mobile elements. Here, we disclose pipolins, previously found bacterial phyla encoding a primer-independent PolB, as new category widespread defense islands. The analysis the occurrence structure pipolins revealed that they are commonly integrative elements flanked by direct repeats Gammaproteobacteria genomes, mainly Escherichia, Vibrio or Aeromonas, often taking up known integration hotspots. Remarkably, integrase dynamics correlates with alternative spots enables lifestyles, from to mobilizable plasmid such members genera Limosilactobacillus, Pseudosulfitobacter Staphylococcus. Pipolins harbor minimal core large cargo module enriched for factors. In addition, weighted gene repertoire relatedness many these factors actively exchanged These findings indicate and, potentially islands, act orthogonal reservoirs genes, transferable immune autonomous MGEs, suggesting complementary exchange mechanisms populations.

Language: Английский

Citations

1
Pipolins are bimodular platforms that maintain a reservoir of defense systems exchangeable with various bacterial genetic mobile elements DOI Creative Commons

Víctor Mateo-Cáceres,

Modesto Redrejo‐Rodríguez

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 22, 2024

ABSTRACT Defense genes gather in diverse types of genomic islands bacteria and provide immunity against viruses other genetic mobile elements. Here, we disclose pipolins, previously found bacterial phyla encoding a primer-independent PolB, as new category widespread defense islands. The analysis the occurrence structure pipolins revealed that they are commonly integrative elements flanked by direct repeats Gammaproteobacteria genomes, mainly Escherichia , Vibrio or Aeromonas often taking up known integration hotspots. Remarkably, integrase dynamics correlates with alternative spots enables lifestyles, from to mobilizable plasmid such members genera Limosilactobacillus Pseudosulfitobacter Staphylococcus . Pipolins harbor minimal core large cargo module enriched for factors. In addition, weighted gene repertoire relatedness many these factors actively exchanged These findings indicate and, potentially islands, act orthogonal reservoirs genes, transferable immune autonomous MGEs, suggesting complementary exchange mechanisms populations.

Language: Английский

Citations

0
The defensome of Acinetobacter baumannii reveals two genome groups according to their innate defense systems and phage profile DOI Creative Commons
Antonio Moreno-Rodríguez, Alejandro Rubio, Andrés Garzón

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 27, 2024

Abstract Phages are guilty of killing daily almost half bacterial cells, while bacteria have developed defense mechanisms that number in the dozens. Individual systems gained and lost by genomes same species, depending on their fitness advantage. Thus, some a certain combination systems, other act as reservoir for rest thus constituting so-called pan-immune system species. Here we analyzed thousands bacterium Acinetobacter baumannii , an opportunistic pathogen humans great clinical concern, found 81 different heterogeneously distributed. By analyzing how these combine, more than lack universal DNA-methylating restriction-modification (R-M) harbor alternative innate SspBCDE performs DNA phosphorothioate modification. In addition, adaptive CRISPR-Cas could synergistically to R-M based frequency co-appearance. The presence one or modulate evolution this causing them present profile phages integrated into genome. We also observed many is associated with higher prophages, which be due fact prophage carries system, would not need environments where phage absent.

Language: Английский

Citations

0