DNA Insecticide: An Emerging Crop Protection Technology DOI
Hemant Kumar, Sweta Verma,

Rakesh Kumar Behera

et al.

Indian Journal of Entomology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 7, 2024

The agriculture industry faces a challenge in balancing the need for pest management and environmental protection. This review describes DNA insecticides, composed of small, single-stranded oligonucleotides that are environment-friendly target pests efficiently specifically insecticide stems from discovery coevolution between baculo viruses insects, where virus exploit inhibitors apoptosis (IAPs) genes to stop insect-induced apoptosis. Historically, journey started by targeting IAP genes. Butthis context has now changed as insecticides work best ribosomal RNAs (rRNA) insect pests, oligonucleotide rRNAs or any gene can be artificially designed using Contact Unmodified Antisense (CUAD) Biotechnology against host being operative nature got later uncovered humans showcases novel, advantageous, secure approach manage pests.

Language: Английский

Contact unmodified antisense DNA (CUAD) biotechnology: list of pest species successfully targeted by oligonucleotide insecticides DOI Creative Commons

Vladimir V. Oberemok,

Kateryna V. Laikova, Nikita V. Gal’chinsky

et al.

Frontiers in Agronomy, Journal Year: 2024, Volume and Issue: 6

Published: May 27, 2024

OPINION article Front. Agron., 27 May 2024Sec. Pest Management Volume 6 - 2024 | https://doi.org/10.3389/fagro.2024.1415314

Citations

7
Advances in Antisense Oligo Technology for Sustainable Crop Protection DOI
V. D. Patil, Sumit Jangra, Amalendu Ghosh

et al.

Critical Reviews in Plant Sciences, Journal Year: 2024, Volume and Issue: 43(6), P. 405 - 427

Published: Aug. 28, 2024

Since its inception, antisense oligonucleotides (ASOs) have been extensively used in functional genomics. Recent advancements chemical modification and delivery technology extend applications to the targeted management of crop pests. ASO was conceptualized early 1970s first applied inhibit retrovirus replication chickens. The principles ASOs rely on target complementarity by hybridizing with mRNA inhibiting function. However, application received little attention because high instability biological environment. Initial ASOs, featuring phosphorothioate linkages, laid groundwork for subsequent advancements, addressing challenges such as nuclease degradation cellular uptake limitations. second generation introduced 2'-modifications enhance stability specificity while allowing reversible gene regulation. Third-generation incorporated innovative modifications like locked nucleic acids (LNAs), peptide (PNAs), phosphoramidate morpholino oligomers (PMOs), 2′-deoxy-2′-fluoro-d-arabinonucleic acid (FANA), cyclohexene (CeNAs), tricyclo-DNA (tcDNA), offering enhanced efficacy stability. continues evolve, promising precision medicine customized therapies genetic diseases. In agriculture, selectively essential genes pests or pathogens, which would be a novel option providing precise protection reducing reliance conventional pesticides. studies demonstrated successful thrips, psyllids, Gypsy moths, scale insects, citrus greening, Zebra-chip Additionally, hold potential improving yield quality modulating expression related plant growth, development, stress responses. They can abiotic tolerance, regulate associated specific traits, offer alternative strategies managing diseases, targeting S-genes tomatoes resistance against bacterial spots. These antecede oligo-based pesticides that will revolutionize future agriculture pesticide uses harmful impacts ecosystem. Further environmental stability, field efficacy, formulation, safety mold this into tool sustainable protection. This review highlights fundamentals ASO, recent design chemistry, systems, validation optimization process, pest management. serve ready reckoner research application.

Language: Английский

Citations

3
CUAD (Contact Unmodified Antisense DNA) Biotechnology: List of Pest Species Successfully Targeted by Oligonucleotide Insecticides DOI Open Access

Vladimir Oberemok,

Katerina Laikova,

Nikita V. Gal’chinsky

et al.

Published: March 26, 2024

The idea of CUAD (contact unmodified antisense DNA) biotechnology and the use oligonucleotide insecticides (briefly, olinscides, or DNA insecticides) was proposed on lepidopterans gradually disseminated hemipteran pests. As next-generation platform for creation insecticides, it has a number unique characteristics (short as contact insecticide, insect rRNAs target, containment mechanism action) that distinguish from existing classes chemical insecticides. Oligonucleotide possess low carbon footprint, high safety non-target organisms, rapid biodegradability, avoidance target-site resistance, ensuring prosperity agricultural countries will implement this innovation. class olinscides create opportunities have never existed before in crop protection. Using flexible platform, any farmer, botanical garden pest control company is capable creating its own arsenal well-tailored particular population pest. Most technological innovations start out very expensive don't work well, but eventually working well become cheap. come long way 16 years close to point be implemented large scale against certain groups In article we provide basic knowledge pesticides list pests (mainly suborder Sternorrhyncha, Hemiptera) which were successfully controlled by us recently.

Language: Английский

Citations

1
Potent and selective 'genetic zipper' method for plant protection: innovative DNA psyllidicides against Trioza alacris Flor based on short unmodified antisense oligonucleotides targeting rRNA of the pest DOI Creative Commons
Volodymyr V. Oberemok, Ilya Novikov, Ekaterina Yatskova

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 2, 2024

Abstract Chemical insecticides increased the chemical burden on natural ecosystems posing environmental health risk factor. The urgent need for a more sustainable and ecological approach has produced many innovative ideas, including eco-friendly ‘genetic zipper’ method (or CUAD platform) based contact oligonucleotide insecticides. Oligonucleotide have enjoyed success recently sternorrhynchans showing highly adaptable structure distinct insect pest species selective mode of action. In this article, we describe efficiency (briefly, olinscides or DNA insecticides) Alacris-11 Laura-11, as well their combined use in mixture (1:1), designed control bay sucker ( Trioza alacris Flor), an important psyllid noble laurel Laurus nobilis L.). These are short unmodified antisense oligonucleotides that target ITS2 between 5.8S rRNA 28S pre-rRNA (Laura-11) region mature (Alacris-11). maximum mortality, observed 14 th day experiment, comprised 95.01 ± 4.42 % Alacris-11, 97.16 2.48 98.72 1.14 (1:1). CTGA-11 did not cause any significant mortality (9.38 0.57 %), emphasizing selectivity action results show potent specific nature opens up new frontiers economically psyllids agriculture forestry, Asian citrus Diaphorina citri Kuwayama) others. Scientists can easily adopt plethora pests because is programmable molecule provides game-changing characteristics plant projection.

Language: Английский

Citations

0
rRNA-specific antisense DNA and dsDNA trigger rRNA biogenesis and cause potent insecticidal effect on insect pestCoccus hesperidumL. DOI Creative Commons
Volodymyr V. Oberemok, Nikita V. Gal’chinsky, Ilya Novikov

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

Abstract As a completely new principle and active substance for plant protection, unmodified DNA was shown to function as contact insecticide in 2008. CUAD (contact antisense DNA) biotechnology is built on the use of short oligonucleotides insect pest control. Being novel class insecticides, oligonucleotide insecticides target rRNAs and/or pre-rRNAs recently showed high insecticidal potential against sap-feeding pests, main vectors viruses one most economically-damaging groups herbivorous insects. In order all possible opportunities biotechnology, this article we investigated 11-mer oligos comparison with long 56-mer single-stranded double-stranded sequences Coccus hesperidum control found lower efficiency latter. Also show that containment (DNAc) mechanism, sternorrhynchans, represents interesting important cell life interplay between different types oligos. course DNAc, hypercompensation rRNA triggered by highly somewhat complementary but more pronounced later degradation significant mortality seen only case perfect complementarity rRNA. Oligonucleotide are effective safe agents sternorrhynchans other easy ‘tune’ particular crops also flexible instantly re-create target-site resistance. Minimalist approach, dissolved water, so potent selective eco-friendly innovation reveals entirely dimension protection – DNA-programmable Fundamentally important, surprising results reveal regulation expression via interaction viral DNA. Significance Statement Using platform any farmer, botanical garden or company capable creating its own unique arsenal well-tailored population pest. Most technological innovations start out very expensive don’t work well, eventually working well become cheap. close point be implemented at affordable price certain pests large scale. Results provide knowledge about host sheds light

Language: Английский

Citations

0
DNA Insecticide: An Emerging Crop Protection Technology DOI
Hemant Kumar, Sweta Verma,

Rakesh Kumar Behera

et al.

Indian Journal of Entomology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 7, 2024

The agriculture industry faces a challenge in balancing the need for pest management and environmental protection. This review describes DNA insecticides, composed of small, single-stranded oligonucleotides that are environment-friendly target pests efficiently specifically insecticide stems from discovery coevolution between baculo viruses insects, where virus exploit inhibitors apoptosis (IAPs) genes to stop insect-induced apoptosis. Historically, journey started by targeting IAP genes. Butthis context has now changed as insecticides work best ribosomal RNAs (rRNA) insect pests, oligonucleotide rRNAs or any gene can be artificially designed using Contact Unmodified Antisense (CUAD) Biotechnology against host being operative nature got later uncovered humans showcases novel, advantageous, secure approach manage pests.

Language: Английский

Citations

0