A Comparative Roadmap of PIWI-Interacting RNAs (piRNAs) Across Seven Species Reveals Insights Into de novo piRNA Precursor Formation in Mammals DOI
Παρθένα Κωνσταντινίδου, Zuzana Loubalová,

Franziska Ahrend

et al.

Published: Jan. 1, 2024

PIWI-interacting RNAs (piRNAs) play a crucial role in safeguarding genome integrity by silencing mobile genetic elements. From flies to human, piRNAs originate from long single-stranded precursors encoded genomic piRNA clusters. How clusters form adapt novel invaders and evolve maintain protection, remain key outstanding questions. Here, we generated roadmap of across seven species that highlights both similarities variations. In mammals, identified transcriptional readthrough as mechanism generate transposon insertions downstream-of-genes (piC-DoGs). Together with the well-known stress-dependent DoG-transcripts, our findings suggest molecular for formation response retroviral invasion. Finally, class dynamic humans, underscoring unique features human germ cell biology. Our results advance understanding conserved principles species-specific variations biology provide tools future studies.

Language: Английский

Dynamic co-evolution of transposable elements and the piRNA pathway in African cichlid fishes DOI Creative Commons
Miguel Vasconcelos Almeida, Moritz Blumer, Chengwei Ulrika Yuan

et al.

Genome biology, Journal Year: 2025, Volume and Issue: 26(1)

Published: Jan. 22, 2025

Abstract Background East African cichlid fishes have diversified in an explosive fashion, but the (epi)genetic basis of phenotypic diversity these remains largely unknown. Although transposable elements (TEs) been associated with variation cichlids, little is known about their transcriptional activity and epigenetic silencing. We set out to bridge this gap understand interactions between TEs hosts. Results Here, we describe dynamic patterns TE expression gonads during early development. Orthology inference revealed strong conservation silencing factors expansion piwil1 genes Lake Malawi likely driven by PiggyBac TEs. The expanded copies signatures positive selection retain amino acid residues essential for catalytic activity. Furthermore, cichlids express a Piwi-interacting RNA (piRNA) pathway that targets define genomic sites piRNA production find divergence closely related species, line fast evolution piRNA-producing loci. Conclusions Our findings suggest co-evolution host pathways radiations. propose has contributed diversity.

Language: Английский

Citations

0
A comparative roadmap of PIWI-interacting RNAs across seven species reveals insights into de novo piRNA-precursor formation in mammals DOI Creative Commons
Παρθένα Κωνσταντινίδου, Zuzana Loubalová,

Franziska Ahrend

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(10), P. 114777 - 114777

Published: Sept. 19, 2024

Language: Английский

Citations

3
A Comparative Roadmap of PIWI-Interacting RNAs (piRNAs) Across Seven Species Reveals Insights Into de novo piRNA Precursor Formation in Mammals DOI
Παρθένα Κωνσταντινίδου, Zuzana Loubalová,

Franziska Ahrend

et al.

Published: Jan. 1, 2024

PIWI-interacting RNAs (piRNAs) play a crucial role in safeguarding genome integrity by silencing mobile genetic elements. From flies to human, piRNAs originate from long single-stranded precursors encoded genomic piRNA clusters. How clusters form adapt novel invaders and evolve maintain protection, remain key outstanding questions. Here, we generated roadmap of across seven species that highlights both similarities variations. In mammals, identified transcriptional readthrough as mechanism generate transposon insertions downstream-of-genes (piC-DoGs). Together with the well-known stress-dependent DoG-transcripts, our findings suggest molecular for formation response retroviral invasion. Finally, class dynamic humans, underscoring unique features human germ cell biology. Our results advance understanding conserved principles species-specific variations biology provide tools future studies.

Language: Английский

Citations

1