A review of virus host factor discovery using CRISPR screening DOI Creative Commons

W. See,

Meisam Yousefi, Yaw Shin Ooi

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(11)

Published: Oct. 18, 2024

ABSTRACT The emergence of genome-scale forward genetic screening techniques, such as Haploid Genetic screen and clustered regularly interspaced short palindromic repeats (CRISPR) knockout has opened new horizons in our understanding virus infection biology. CRISPR become a popular tool for the discovery novel host factors several viruses due to its specificity efficiency genome editing. Here, we review how revolutionized virus-host interactions from scientific technological viewpoints. A summary published screens conducted thus far uncover is presented, highlighting their experimental design significant findings. We will outline relevant methods customizing process answer more specific hypotheses compile glossary show aspects. Furthermore, using flaviviruses severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) examples, hope offer broad-based perspective on capabilities serve reference point guide future unbiased factors.

Language: Английский

A review of virus host factor discovery using CRISPR screening DOI Creative Commons

W. See,

Meisam Yousefi, Yaw Shin Ooi

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(11)

Published: Oct. 18, 2024

ABSTRACT The emergence of genome-scale forward genetic screening techniques, such as Haploid Genetic screen and clustered regularly interspaced short palindromic repeats (CRISPR) knockout has opened new horizons in our understanding virus infection biology. CRISPR become a popular tool for the discovery novel host factors several viruses due to its specificity efficiency genome editing. Here, we review how revolutionized virus-host interactions from scientific technological viewpoints. A summary published screens conducted thus far uncover is presented, highlighting their experimental design significant findings. We will outline relevant methods customizing process answer more specific hypotheses compile glossary show aspects. Furthermore, using flaviviruses severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) examples, hope offer broad-based perspective on capabilities serve reference point guide future unbiased factors.

Language: Английский

Citations

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