Understanding the genetics of sex determination in insects and its relevance to genetic pest management DOI Creative Commons
Jade S. Ashmore, Bernard Slippers, Tuan A. Duong

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Abstract Sex determination pathways regulate male and female‐specific development differentiation offer potential targets for genetic pest management methods. Insect sex are comprised of primary signals, relay genes terminal genes. Primary signals coleopteran, dipteran, hymenopteran lepidopteran species highly diverse the sex‐specific splicing based on signal dosage, amino acid composition or interaction with paternally inherited In coleopterans, hymenopterans some dipterans, Transformer orthologs from serine‐arginine protein family that Alternative in dipterans lack lepidopterans. Doublesex Fruitless conserved zinc‐finger proteins expression downstream influencing physical traits courtship behaviours a manner. Genetic methods can use different mechanisms to exploit disrupt regions Female‐specific be exploited produce lethal gene only females disrupted impede female fertility. Reducing number fertile populations creates male‐biased ratio eventually leads local elimination population. Knowledge basis is important enable these management.

Language: Английский

Engineering drive-selection balance for localised population suppression with neutral dynamics DOI Creative Commons
Katie Willis, Austin Burt

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 21, 2024

Abstract Whilst the release of sterile males has been highly successful in suppressing some pest populations, it is impractical for other species due to disappearing after a single generation, necessitating large, repeated releases maintain sufficient impact. Synthetic gene drives promise more efficient approaches since they can increase frequency from rare, yet this also allows them spread across landscape, which may not always be desired. Between these two extremes are selectively neutral genetic constructs persist at released, offering potential suppression that remains localised. One way achieve would have perfect balance, all construct frequencies, between drive increasing and selection decreasing it. Here we describe create balance involving toxin-antidote causes recessive lethality, encodes genomic editor makes dominant lethal edits genome, provides protection against action or consequences editing. Computer modelling shows design 100-fold than males, 1000-fold when released alongside booster. We designs CRISPR-based molecular construction, including options avoid using recoded genes as antidotes.

Language: Английский

Citations

2
Gene drive-based population suppression in the malaria vectorAnopheles stephensi DOI Creative Commons
Xuejiao Xu,

Jingheng Chen,

You Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 24, 2024

Abstract Gene drives are alleles that can bias the inheritance of specific traits in target populations for purpose modification or suppression. Here, we constructed a homing suppression drive major urban malaria vector Anopheles stephensi targeting female-specific exon doublesex , incorporating two gRNAs and nanos- Cas9 promoter to reduce functional resistance improve female heterozygote fitness. Our result showed was recessive sterile both females males, with various intersex phenotypes homozygotes. Both male heterozygotes only moderate conversion, indicating nos has lower activity A. than gambiae . By amplicon sequencing, detected very low level allele formation. Combination vasa -Cas9 line demonstrated boost conversion rate 100%, suggesting use similar systems population continuous release strategy SIT fRIDL techniques. This study contributes valuable insights development more efficient environmentally friendly pest control tools aimed at disrupting disease transmission.

Language: Английский

Citations

2
Understanding the genetics of sex determination in insects and its relevance to genetic pest management DOI Creative Commons
Jade S. Ashmore, Bernard Slippers, Tuan A. Duong

et al.

Insect Molecular Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 31, 2024

Abstract Sex determination pathways regulate male and female‐specific development differentiation offer potential targets for genetic pest management methods. Insect sex are comprised of primary signals, relay genes terminal genes. Primary signals coleopteran, dipteran, hymenopteran lepidopteran species highly diverse the sex‐specific splicing based on signal dosage, amino acid composition or interaction with paternally inherited In coleopterans, hymenopterans some dipterans, Transformer orthologs from serine‐arginine protein family that Alternative in dipterans lack lepidopterans. Doublesex Fruitless conserved zinc‐finger proteins expression downstream influencing physical traits courtship behaviours a manner. Genetic methods can use different mechanisms to exploit disrupt regions Female‐specific be exploited produce lethal gene only females disrupted impede female fertility. Reducing number fertile populations creates male‐biased ratio eventually leads local elimination population. Knowledge basis is important enable these management.

Language: Английский

Citations

1