Global organelle profiling reveals subcellular localization and remodeling at proteome scale DOI Creative Commons
Marco Y. Hein, Duo Peng, Verina Todorova

et al.

Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Defining the subcellular distribution of all human proteins and their remodeling across cellular states remains a central goal in cell biology. Here, we present high-resolution strategy to map organization using organelle immunocapture coupled mass spectrometry. We apply this workflow cell-wide collection membranous membraneless compartments. A graph-based analysis assigns localization over 7,600 proteins, defines spatial networks, uncovers interconnections between Our approach can be deployed comprehensively profile proteome during perturbation. By characterizing landscape following HCoV-OC43 viral infection, discover that many are regulated by changes rather than abundance. results establish proteome-wide provides key insights for elucidating responses, uncovering an essential role ferroptosis OC43 infection. dataset explored at organelles.czbiohub.org.

Language: Английский

TRPML1 activation ameliorates lysosomal phenotypes in CLN3 deficient retinal pigment epithelial cells DOI Creative Commons

Daniela Wünkhaus,

R. Tang, Kwamina Nyame

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 29, 2024

Mutations in the lysosomal membrane protein CLN3 cause Juvenile Neuronal Ceroid Lipofuscinosis (JNCL). Activation of ion channel TRPML1 has previously been shown to be beneficial several neurodegenerative disease models. Here, we tested whether activation rescues disease-associated phenotypes CLN3-deficient retinal pigment epithelial (ARPE-19 CLN3-KO) cells. ARPE-19 CLN3-KO cells accumulate LAMP1 positive organelles and show storage mitochondrial ATPase subunit C (SubC), globotriaosylceramide (Gb3), glycerophosphodiesters (GPDs), whereas bis(monoacylglycero)phosphate (BMP/LBPA) lipid levels were significantly decreased. reduced Gb3 SubC but failed restore BMP TRPML1-mediated decrease was TFEB-independent, identified enhanced exocytosis as a likely mechanism for clearing including GPDs. Therefore, represent human cell model showing many described core deficits, some which can improved using agonists.

Language: Английский

Citations

4
Mechanisms and functions of lysosomal lipid homeostasis DOI Creative Commons
Michael Ebner, Florian Fröhlich, Volker Haucke

et al.

Cell chemical biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Lack of significant ganglioside changes in Slc17a5 heterozygous mice: Relevance to FSASD and Parkinson's disease DOI
Marya S. Sabir,

Mahin Hossain,

Laura Pollard

et al.

Biochemistry and Biophysics Reports, Journal Year: 2025, Volume and Issue: 42, P. 101979 - 101979

Published: March 14, 2025

Language: Английский

Citations

0
Mitochondria-organelle crosstalk in establishing compartmentalized metabolic homeostasis DOI
Brandon Chen, Costas A. Lyssiotis, Yatrik M. Shah

et al.

Molecular Cell, Journal Year: 2025, Volume and Issue: 85(8), P. 1487 - 1508

Published: April 1, 2025

Language: Английский

Citations

0
Global organelle profiling reveals subcellular localization and remodeling at proteome scale DOI Creative Commons
Marco Y. Hein, Duo Peng, Verina Todorova

et al.

Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Defining the subcellular distribution of all human proteins and their remodeling across cellular states remains a central goal in cell biology. Here, we present high-resolution strategy to map organization using organelle immunocapture coupled mass spectrometry. We apply this workflow cell-wide collection membranous membraneless compartments. A graph-based analysis assigns localization over 7,600 proteins, defines spatial networks, uncovers interconnections between Our approach can be deployed comprehensively profile proteome during perturbation. By characterizing landscape following HCoV-OC43 viral infection, discover that many are regulated by changes rather than abundance. results establish proteome-wide provides key insights for elucidating responses, uncovering an essential role ferroptosis OC43 infection. dataset explored at organelles.czbiohub.org.

Language: Английский

Citations

2