A human induced pluripotent stem (hiPS) cell model for the holistic study of epithelial to mesenchymal transitions (EMTs) DOI Creative Commons

Caroline Hookway,

Antoine Borensztejn,

Leigh K. Harris

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

The epithelial to mesenchymal transition (EMT) is a widely studied but poorly defined state change due the variety of ways in which it has been characterized cells. There need for reproducible cell model systems that enable integration and comparison different types measured observations cells across many distinct cellular contexts. We present human induced pluripotent stem (hiPS) as such system by demonstrating its utility through comparative analysis hiPS cell-EMT 2D 3D culture geometries. developed live-imaging-based assays directly compare examples changes function (via migration timing), molecular components expression marker proteins), organization reorganization junctions), environment dynamics basement membrane) same experimental system. EMT-related we occurred earlier colonies than lumenoids, likely differences membrane environments associated with vs. initial have made 449 60-hour-long time-lapse movies tools used visualization open-source easily accessible resource future work this field.

Language: Английский

A human induced pluripotent stem (hiPS) cell model for the holistic study of epithelial to mesenchymal transitions (EMTs) DOI Creative Commons

Caroline Hookway,

Antoine Borensztejn,

Leigh K. Harris

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

The epithelial to mesenchymal transition (EMT) is a widely studied but poorly defined state change due the variety of ways in which it has been characterized cells. There need for reproducible cell model systems that enable integration and comparison different types measured observations cells across many distinct cellular contexts. We present human induced pluripotent stem (hiPS) as such system by demonstrating its utility through comparative analysis hiPS cell-EMT 2D 3D culture geometries. developed live-imaging-based assays directly compare examples changes function (via migration timing), molecular components expression marker proteins), organization reorganization junctions), environment dynamics basement membrane) same experimental system. EMT-related we occurred earlier colonies than lumenoids, likely differences membrane environments associated with vs. initial have made 449 60-hour-long time-lapse movies tools used visualization open-source easily accessible resource future work this field.

Language: Английский

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