EBAX-1/ZSWIM8 destabilizes miRNAs resulting in transgenerational memory of a predatory trait DOI Creative Commons
Shiela Pearl Quiobe, Ata Kalirad, Waltraud Röseler

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 14, 2024

Abstract Environmental influences on traits and associated transgenerational epigenetic inheritance have widespread implications, but remain controversial underlying mechanisms poorly understood. We introduce long-term environmental induction experiments alternative diets in Pristionchus pacificus , a nematode exhibiting mouth-form plasticity including predation, by propagating 110 isogenic lines for 101 generations with food-reversal experiments. found dietary subsequent memory of the predatory morph identified role ubiquitin ligase EBAX-1/ZSWIM8 this process. Ppa-ebax-1 mutants no Ppa- EBAX-1 destabilizes clustered microRNA family miR-2235a/miR-35. Deletions cluster 44 identical miR-2235a copies resulted precocious extended morph. These findings indicate that miRNAs resulting memory, suggesting target-directed miRNA degradation.

Language: Английский

EBAX-1/ZSWIM8 destabilizes miRNAs, resulting in transgenerational inheritance of a predatory trait DOI Creative Commons
Shiela Pearl Quiobe, Ata Kalirad, Waltraud Röseler

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(11)

Published: March 12, 2025

Environmental influences on traits and associated transgenerational epigenetic inheritance have widespread implications but remain controversial underlying mechanisms poorly understood. We introduce long-term environmental induction experiments alternative diets in Pristionchus pacificus , a nematode exhibiting mouth-form plasticity including predation, by propagating 110 isogenic lines for 101 generations with food-reversal experiments. found dietary subsequent of the predatory morph identified role ubiquitin ligase EBAX-1/ZSWIM8 this process. Ppa-ebax-1 mutants are defective, Ppa- EBAX-1 destabilizes clustered microRNA family miR-2235a/miR-35. Deletions cluster 44 identical miR-2235a copies resulted precocious extended morph. These findings indicate that miRNAs, resulting inheritance, suggesting target-directed miRNA degradation.

Language: Английский

Citations

0
The Maternal Effect of SKN-1B and DAF-7 on Intergenerational Pathogen Avoidance Learning inC. elegans DOI Creative Commons
Rebekka Paisner,

Mattias Bekele,

Ebubechi Onyia

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

Abstract Parental health strongly influences the development of embryos, and can ultimately influence behavior post-birth. When exposed to pathogenic Pseudomonas aeruginosa (PA14), C. elegans produces a robust immune response, as well learned avoid PA14 in future. This avoidance be inherited by F1 generation. An important trigger worm’s response infection is Nrf2 homologue, SKN-1. We find that SKN-1B isoform intergenerational inheritance animals via DAF-7 (TGF-β) signaling from ASI. Functional required both P0 generations facilitate due parental exposure. While was not observed F2+ generations, single generation learning investigated here may provide parallel pathway improve fitness dynamic environment.

Language: Английский

Citations

0
Absence of Evidence is Not Evidence of Absence: The many flaws in the case against transgenerational epigenetic inheritance of pathogen avoidance inC. elegans DOI Open Access
Rachel Kaletsky, Rebecca S. Moore, Titas Sengupta

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 7, 2024

Abstract After examining the data and methods presented in Gainey, et al., bioRxiv , 2024 1 we conclude that authors did not use an experimental paradigm would have allowed them to replicate our results on transgenerational epigenetic inheritance (TEI) of learned avoidance regularly observe 2–5 . That is, agree with their experiments show no evidence TEI. However, there are substantial differences execution every step work also make it impossible for claim they replicating or protocols. Based these differences, do believe is issue “robustness” “reliability” TEI findings, but rather Hunter colleagues fact tested central condition - small RNA production by bacteria subsequent uptake C. elegans nor carried out proper behavioral imaging assays assess this behavior. Our shows indeed example just observed laboratory settings PA14, induced wild strains Pseudomonas exhibiting its robustness. Just as offered advice training colleagues, happy advise anyone who wishes learn assay. We now performed absence azide, a deviation from protocol deliberately made, found omission may account most if all results. It disingenuous used protocol, given chose same conditions basic assay work, chemotaxis assay, which been necessary order reproduce work. Therefore, Gainey al.’s claims “irreproducible” supported evidence.

Language: Английский

Citations

3
EBAX-1/ZSWIM8 destabilizes miRNAs resulting in transgenerational memory of a predatory trait DOI Creative Commons
Shiela Pearl Quiobe, Ata Kalirad, Waltraud Röseler

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 14, 2024

Abstract Environmental influences on traits and associated transgenerational epigenetic inheritance have widespread implications, but remain controversial underlying mechanisms poorly understood. We introduce long-term environmental induction experiments alternative diets in Pristionchus pacificus , a nematode exhibiting mouth-form plasticity including predation, by propagating 110 isogenic lines for 101 generations with food-reversal experiments. found dietary subsequent memory of the predatory morph identified role ubiquitin ligase EBAX-1/ZSWIM8 this process. Ppa-ebax-1 mutants no Ppa- EBAX-1 destabilizes clustered microRNA family miR-2235a/miR-35. Deletions cluster 44 identical miR-2235a copies resulted precocious extended morph. These findings indicate that miRNAs resulting memory, suggesting target-directed miRNA degradation.

Language: Английский

Citations

0