Methanogenesis inhibition remodels microbial fermentation and stimulates acetogenesis in ruminants DOI Creative Commons
Gaofeng Ni, Nicola Walker, André Fischer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 15, 2024

Abstract Rumen microbiota enable ruminants to grow on fibrous plant materials but also produce methane, driving 5% of global greenhouse gas emissions and leading a loss gross energy content. Methanogenesis inhibitors such as 3-nitrooxypropanol (3-NOP) decrease methane in when supplemented feed. Yet we lack system-wide, species-resolved understanding how the rumen remodels following inhibition this influences animal production. Here, conducted large-scale trial with 51 dairy calves analyse responses 3-NOP, pairing host performance, emissions, nutritional profiles genome-resolved metagenomic metatranscriptomic data. 3-NOP supplementation decreased by an average 62%, modulated short-chain fatty acid H 2 levels, did not affect dietary intake or performance. We created microbial genome catalogue unprecedented mapping rate. observed strong reduction methanogens stimulation reductive acetogens, primarily novel uncultivated lineages Candidatus Faecousia. However, there was shift major fermentative communities away from acetate production response hydrogen accumulation. Thus, divergent hydrogenotrophic limit potential productivity gains reduction. Reporting one largest reductions field date, study links ruminant specific species. These findings emphasise importance microbiota-wide analysis for optimising mitigation strategies identify promising simultaneously reduce while increasing Significance Statement One strategy increase sustainability livestock is modulate absorbable nutrients rather than potent methane. Previous studies show supplementing feed methanogenesis reduces leads inconsistent gains. Here report definitive trial, combining data, meta-omics, structural modelling, resolve key microbes pathways controlling nutrient ruminants. that shifts composition gene expression hydrogen-cycling offer insights at resolution, data analytical framework provide valuable resources develop solutions enhance sustainability.

Language: Английский

Methanogenesis inhibition remodels microbial fermentation and stimulates acetogenesis in ruminants DOI Creative Commons
Gaofeng Ni, Nicola Walker, André Fischer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 15, 2024

Abstract Rumen microbiota enable ruminants to grow on fibrous plant materials but also produce methane, driving 5% of global greenhouse gas emissions and leading a loss gross energy content. Methanogenesis inhibitors such as 3-nitrooxypropanol (3-NOP) decrease methane in when supplemented feed. Yet we lack system-wide, species-resolved understanding how the rumen remodels following inhibition this influences animal production. Here, conducted large-scale trial with 51 dairy calves analyse responses 3-NOP, pairing host performance, emissions, nutritional profiles genome-resolved metagenomic metatranscriptomic data. 3-NOP supplementation decreased by an average 62%, modulated short-chain fatty acid H 2 levels, did not affect dietary intake or performance. We created microbial genome catalogue unprecedented mapping rate. observed strong reduction methanogens stimulation reductive acetogens, primarily novel uncultivated lineages Candidatus Faecousia. However, there was shift major fermentative communities away from acetate production response hydrogen accumulation. Thus, divergent hydrogenotrophic limit potential productivity gains reduction. Reporting one largest reductions field date, study links ruminant specific species. These findings emphasise importance microbiota-wide analysis for optimising mitigation strategies identify promising simultaneously reduce while increasing Significance Statement One strategy increase sustainability livestock is modulate absorbable nutrients rather than potent methane. Previous studies show supplementing feed methanogenesis reduces leads inconsistent gains. Here report definitive trial, combining data, meta-omics, structural modelling, resolve key microbes pathways controlling nutrient ruminants. that shifts composition gene expression hydrogen-cycling offer insights at resolution, data analytical framework provide valuable resources develop solutions enhance sustainability.

Language: Английский

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