The Drosophila maternal-effect gene abnormal oocyte (ao) does not repress histone gene expression DOI Creative Commons
Risa Takenaka,

Sierra M. Simmerman,

Casey A. Schmidt

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

The abnormal oocyte ( ao ) gene of Drosophila melanogaster is a maternal-effect lethal previously identified as encoding transcriptional regulator core histones. However, background genetic mutations in existing mutant strains could compromise their utility manipulating histone levels. To distinguish the true phenotype from effects, we created two new reagents: CRISPR/Cas9-mediated knockout allele for and molecular analyses an epitope-tagged cytological experiments. Using these reagents, confirm previous findings that exhibits lethality, which can be rescued by either decrease copy number or Y chromosome heterochromatin. We also Ao protein localizes to locus bodies ovaries. Our data suggest genetically interacts with genes heterochromatin, suggested. contrary prior findings, find does not repress transcript Thus, basis -associated lethality remains unknown.

Language: Английский

Cis element length variability does not confer differential transcription factor occupancy at the D. melanogaster histone locus DOI
Lauren J. Hodkinson, Leila E. Rieder

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 28, 2024

Histone genes require precise regulation to maintain histone homeostasis and ensure nucleosome stoichiometry. Animal often have unique clustered genomic organization. However, there is variability of gene number organization as well differential the across species. The

Language: Английский

Citations

1
Genome structural variants shape adaptive success of an invasive urban malaria vectorAnopheles stephensi DOI Open Access

Alejandra Samano,

Naveen Kumar,

Yi Liao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 30, 2024

Global changes are associated with the emergence of several invasive species. However, genomic determinants adaptive success an species in a new environment remain poorly understood. Genomic structural variants (SVs), consisting copy number variants, play important role adaptation. SVs often cause large shifts ecologically traits, which makes compelling candidates for driving rapid adaptations to environmental changes, is critical success. To address this problem, we investigated

Language: Английский

Citations

1
Cell cycle-regulated transcriptional pausing ofDrosophilareplication-dependent histone genes DOI Creative Commons
James P. Kemp, Mark S. Geisler,

Marie L. Hoover

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Coordinated expression of replication-dependent (RD) histones genes occurs within the Histone Locus Body (HLB) during S phase, but molecular steps in transcription that are cell cycle regulated unknown. We report Drosophila RNA Pol II promotes HLB formation and is enriched outside including G1-arrested cells do not transcribe RD histone genes. In contrast, elongation factor Spt6 HLBs only phase. Proliferating wing eye primordium express full-length mRNAs phase short nascent transcripts G1 or G2 consistent with these being paused then terminated. Full-length produced when Cyclin E/Cdk2 activated as enter Thus, activation by recruitment pol to critical step links gene progression Drosophila.

Language: Английский

Citations

1
Redesigning the Drosophila histone gene cluster: An improved genetic platform for spatiotemporal manipulation of histone function DOI
Aaron T. Crain, Markus Nevil,

Mary Leatham‐Jensen

et al.

Genetics, Journal Year: 2024, Volume and Issue: 228(1)

Published: July 22, 2024

Mutating replication-dependent (RD) histone genes is an important tool for understanding chromatin-based epigenetic regulation. Deploying this in metazoans particularly challenging because RD histones these organisms are typically encoded by many genes, often located at multiple loci. Such gene arrangements make the ability to generate homogenous mutant genotypes site-specific editing quite difficult. Drosophila melanogaster provides a solution problem organized into single large tandem array that can be deleted and replaced with transgenes containing genes. In last ∼15 years several different replacement platforms were developed using simple strategy. However, each platform contains weaknesses preclude full use of powerful developmental genetic capabilities available researchers. Here we describe development newly engineered rectifies weaknesses. We used CRISPR precisely delete (HisC), replacing it multifunctional cassette permits insertion either one or two synthetic arrays selectable markers. designed selectively integrated specific tissues recombinases. also present method rapidly synthesizing any genotype Golden Gate cloning technologies. These improvements facilitate generation cells various stages provide opportunity apply forward strategies interrogate chromatin structure

Language: Английский

Citations

0
The Drosophila maternal-effect gene abnormal oocyte (ao) does not repress histone gene expression DOI Creative Commons
Risa Takenaka,

Sierra M. Simmerman,

Casey A. Schmidt

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

The abnormal oocyte ( ao ) gene of Drosophila melanogaster is a maternal-effect lethal previously identified as encoding transcriptional regulator core histones. However, background genetic mutations in existing mutant strains could compromise their utility manipulating histone levels. To distinguish the true phenotype from effects, we created two new reagents: CRISPR/Cas9-mediated knockout allele for and molecular analyses an epitope-tagged cytological experiments. Using these reagents, confirm previous findings that exhibits lethality, which can be rescued by either decrease copy number or Y chromosome heterochromatin. We also Ao protein localizes to locus bodies ovaries. Our data suggest genetically interacts with genes heterochromatin, suggested. contrary prior findings, find does not repress transcript Thus, basis -associated lethality remains unknown.

Language: Английский

Citations

0