Completing the BASEL phage collection to unlock hidden diversity for systematic exploration of phage–host interactions

PLoS Biology, Journal Year: 2025, Volume and Issue: 23(4), P. e3003063 - e3003063

Published: April 7, 2025

Research on bacteriophages, the viruses infecting bacteria, has fueled development of modern molecular biology and inspired their therapeutic application to combat bacterial multidrug resistance. However, most work so far focused a few model phages which impedes direct applications these findings in clinics suggests that vast potential powerful remained untapped. We have therefore recently composed BASEL collection Escherichia coli (BActeriophage SElection for your Laboratory), made relevant diversity E. K-12 laboratory strain accessible community. These are widely used, but assorted limited by host. now genetically overcome two major limitations K-12, its lack O-antigen glycans presence resident immunity. Restoring expression resulted isolation diverse additional viral groups like Kagunavirus , Nonanavirus Gordonclarkvirinae Gamaleyavirus while eliminating all known antiviral defenses additionally enabled us isolate Wifcevirus genus. Even though some appear be common nature, no from any them had previously been isolated using strains, they thus largely understudied. Overall, 37 new phage isolates added complete collection. were deeply characterized genomically phenotypically with regard host receptors, sensitivity defense systems, range. results highlighted dominant roles barrier recognition restriction-modification systems anticipate completed will propel research phage–host interactions mechanisms, deepening our understanding ecology fostering innovations biotechnology antimicrobial therapy.

Language: Английский

Unlocking hidden phage diversity to complete the BASEL collection for systematic exploration of phage-host interactions

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 12, 2024

Abstract Research on bacteriophages, the viruses infecting bacteria, has fueled development of modern molecular biology and inspired their therapeutic application to combat bacterial multidrug resistance. However, most work so far focused a few model phages which impedes direct applications these findings in clinics suggests that vast potential powerful remained untapped. We have therefore recently composed BASEL collection Escherichia coli (BActeriophage SElection for your Laboratory) made relevant diversity E. K-12 laboratory strain accessible community. These are widely used, but assorted had limited by host. now genetically overcome two major limitations K-12, its lack O-antigen glycans presence resident immunity. Restoring expression resulted isolation diverse additional viral groups like Kagunavirus, Nonanavirus, Gordonclarkvirinae, Gamaleyavirus, while eliminating all known antiviral defenses additionally enabled us isolate Wifcevirus genus. Even though some appear be common nature, no from any them previously been isolated using strains they thus largely understudied. Overall, 37 new phage isolates added complete collection. were deeply characterized genomically phenotypically with regard host receptors, sensitivity defense systems, range. results highlighted dominant roles barrier recognition restriction-modification systems anticipate completed will propel research phage-host interactions mechanisms, deepening our understanding ecology fostering innovations biotechnology antimicrobial therapy.

Language: Английский