The PUF RNA-binding protein, FBF-2, maintains stem cells without binding to RNA DOI Creative Commons
Brian H. Carrick,

Sarah L. Crittenden,

MaryGrace Linsley

et al.

Published: Oct. 25, 2024

Abstract Like all canonical PUF proteins, C. elegans FBF-2 binds to specific RNAs via tripartite recognition motifs (TRMs). Here we report that an mutant protein cannot bind RNA, is nonetheless biologically active and maintains stem cells. This unexpected result challenges the conventional wisdom RBPs must achieve biological activity. Also unexpectedly, interactions with partner proteins can compensate for loss of RNA-binding. only loses activity when its RNA-binding are both defective. These findings highlight complementary contributions protein.

Language: Английский

The PUF RNA-binding protein, FBF-2, maintains stem cells without binding to RNA DOI Creative Commons
Brian H. Carrick,

Sarah L. Crittenden,

MaryGrace Linsley

et al.

Published: Oct. 25, 2024

Abstract Like all canonical PUF proteins, C. elegans FBF-2 binds to specific RNAs via tripartite recognition motifs (TRMs). Here we report that an mutant protein cannot bind RNA, is nonetheless biologically active and maintains stem cells. This unexpected result challenges the conventional wisdom RBPs must achieve biological activity. Also unexpectedly, interactions with partner proteins can compensate for loss of RNA-binding. only loses activity when its RNA-binding are both defective. These findings highlight complementary contributions protein.

Language: Английский

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