A molecular atlas of human granulopoiesis DOI
Christoph Klein, Sebastian Hesse, Jiadong Mao

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Abstract We developed a comprehensive molecular atlas of human granulopoiesis, encompassing the transcriptome (mRNA), proteome, and non-coding RNA (miRNA, lncRNA) seven defined stages neutrophil maturation. Two waves mRNA transcription activity were observed. Whereas first wave, extending from myeloblasts to metamyelocytes, showed correlated transcripts protein translation, second wave was characterized by uncoupling translation. Integrated analysis revealed intricate dynamics translation parallelization in granule subset proteins, ribosomes, mitochondria, respectively. identified hsa-miR-106a-5p family hsa-miR-125a-5p as potential repressors myeloid differentiation myeloblasts, hsa-miR-193a-5p repressor SWI/SNF complex. LncRNA displayed remarkable stage specificity. NEAT1 MAILR expressed only band onwards, ITGB2-AS1 exclusively metamyelocytes. Novel lncRNAs with high expression found terminal S-stage PMN (ENSG00000288819, ENSG00000203644). A factor signatures, including on target genes, previously unrecognized factors well yet underappreciated transcriptional role lactotransferrin (LTF) early maturation stages. Characterization single-cell-clusters via profiles histologically enabled stage-specific cell labeling. To ensure availability our data reproducibility analysis, complete datasets, codes online tool for exploration are available www.granulopoiesis.com.

Language: Английский

A molecular atlas of human granulopoiesis DOI
Christoph Klein, Sebastian Hesse, Jiadong Mao

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Abstract We developed a comprehensive molecular atlas of human granulopoiesis, encompassing the transcriptome (mRNA), proteome, and non-coding RNA (miRNA, lncRNA) seven defined stages neutrophil maturation. Two waves mRNA transcription activity were observed. Whereas first wave, extending from myeloblasts to metamyelocytes, showed correlated transcripts protein translation, second wave was characterized by uncoupling translation. Integrated analysis revealed intricate dynamics translation parallelization in granule subset proteins, ribosomes, mitochondria, respectively. identified hsa-miR-106a-5p family hsa-miR-125a-5p as potential repressors myeloid differentiation myeloblasts, hsa-miR-193a-5p repressor SWI/SNF complex. LncRNA displayed remarkable stage specificity. NEAT1 MAILR expressed only band onwards, ITGB2-AS1 exclusively metamyelocytes. Novel lncRNAs with high expression found terminal S-stage PMN (ENSG00000288819, ENSG00000203644). A factor signatures, including on target genes, previously unrecognized factors well yet underappreciated transcriptional role lactotransferrin (LTF) early maturation stages. Characterization single-cell-clusters via profiles histologically enabled stage-specific cell labeling. To ensure availability our data reproducibility analysis, complete datasets, codes online tool for exploration are available www.granulopoiesis.com.

Language: Английский

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