Fluorescent identification of axons, dendrites and soma of neuronal retinal ganglion cells with a genetic marker as a tool for facilitating the study of neurodegeneration DOI Creative Commons
Puttipong Sripinun,

Wennan Lu,

Sergei Nikonov

et al.

FASEB BioAdvances, Journal Year: 2024, Volume and Issue: 7(1)

Published: Dec. 16, 2024

Abstract This study characterizes a fluorescent Slc17a6 ‐tdTomato neuronal reporter mouse line with strong labeling of axons throughout the optic nerve, retinal ganglion cell (RGC) soma in layer (GCL), and RGC dendrites inner plexiform (IPL). The model facilitated assessment loss models degeneration detection mixed neural/glial cultures. tdTomato signal showed overlap >98% cells immunolabeled markers RBPMS or BRN3A, consistent ubiquitous presence vesicular glutamate transporter 2 (VGUT2, SLC17A6) all subtypes. There was no cross‐labeling ChAT‐positive displaced amacrine GCL, although some emanated from outer layer, horizontal cells. fluorescence allowed rapid screening following nerve crush intraocular pressure (IOP) elevation. bright also enabled non‐invasive monitoring extensive neurite networks neuron/astrocyte interactions culture. Robust Ca 2+ responses to P2X7R agonist BzATP were detected RGCs using ‐indicator Fura‐2. Fluorescence vivo confocal scanning laser ophthalmoscope (cSLO); automatic counts enhanced through machine learning approached numbers found wholemounts. Controls indicated impact expression on light‐dependent function as measured microelectrode array (MEA), structure optical coherence tomography (OCT). In summary, axons, ~all may facilitate RGCs.

Language: Английский

Fluorescent identification of axons, dendrites and soma of neuronal retinal ganglion cells with a genetic marker as a tool for facilitating the study of neurodegeneration DOI Creative Commons
Puttipong Sripinun,

Wennan Lu,

Sergei Nikonov

et al.

FASEB BioAdvances, Journal Year: 2024, Volume and Issue: 7(1)

Published: Dec. 16, 2024

Abstract This study characterizes a fluorescent Slc17a6 ‐tdTomato neuronal reporter mouse line with strong labeling of axons throughout the optic nerve, retinal ganglion cell (RGC) soma in layer (GCL), and RGC dendrites inner plexiform (IPL). The model facilitated assessment loss models degeneration detection mixed neural/glial cultures. tdTomato signal showed overlap >98% cells immunolabeled markers RBPMS or BRN3A, consistent ubiquitous presence vesicular glutamate transporter 2 (VGUT2, SLC17A6) all subtypes. There was no cross‐labeling ChAT‐positive displaced amacrine GCL, although some emanated from outer layer, horizontal cells. fluorescence allowed rapid screening following nerve crush intraocular pressure (IOP) elevation. bright also enabled non‐invasive monitoring extensive neurite networks neuron/astrocyte interactions culture. Robust Ca 2+ responses to P2X7R agonist BzATP were detected RGCs using ‐indicator Fura‐2. Fluorescence vivo confocal scanning laser ophthalmoscope (cSLO); automatic counts enhanced through machine learning approached numbers found wholemounts. Controls indicated impact expression on light‐dependent function as measured microelectrode array (MEA), structure optical coherence tomography (OCT). In summary, axons, ~all may facilitate RGCs.

Language: Английский

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