Spatial transcriptomic analysis of HIV and tuberculosis coinfection in a humanized mouse model reveals specific transcription patterns, immune responses and early morphological alteration signaling DOI Creative Commons
Sitaramaraju Adduri, José Alejandro Bohórquez,

Omoyeni Adejare

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Mycobacterium tuberculosis ( Mtb ) and human immunodeficiency virus (HIV) coinfection is one of the biggest public health concerns worldwide. Both pathogens are adept at modulating immune response and, in case , even inducing structural modification affected tissue. The present study aimed understanding early phenotypical functional changes cell infiltration organ, using a humanized mouse model. mice were infected with either HIV or single infection, both coinfection. Three weeks after lung samples collected, spatial transcriptomics analysis was performed. This revealed high CD4 + T cells but not Coinfected also showed minimal number NK compared to other groups. In addition infection status, histological features influenced pattern lungs. Two distinct airway regions abundance patterns detected by transcriptome profiling. A lymphoid aggregate exhibited transcript profile. cellular architecture did follow seen mature granulomas. However, aggregates granuloma gene expression signatures, pathways associated reactive oxygen species production, oxidative phosphorylation, TGFβ interferon signaling similar specific transcription patterns, responses morphological alteration stage infections.

Language: Английский

Spatial transcriptomic analysis of HIV and tuberculosis coinfection in a humanized mouse model reveals specific transcription patterns, immune responses and early morphological alteration signaling DOI Creative Commons
Sitaramaraju Adduri, José Alejandro Bohórquez,

Omoyeni Adejare

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Mycobacterium tuberculosis ( Mtb ) and human immunodeficiency virus (HIV) coinfection is one of the biggest public health concerns worldwide. Both pathogens are adept at modulating immune response and, in case , even inducing structural modification affected tissue. The present study aimed understanding early phenotypical functional changes cell infiltration organ, using a humanized mouse model. mice were infected with either HIV or single infection, both coinfection. Three weeks after lung samples collected, spatial transcriptomics analysis was performed. This revealed high CD4 + T cells but not Coinfected also showed minimal number NK compared to other groups. In addition infection status, histological features influenced pattern lungs. Two distinct airway regions abundance patterns detected by transcriptome profiling. A lymphoid aggregate exhibited transcript profile. cellular architecture did follow seen mature granulomas. However, aggregates granuloma gene expression signatures, pathways associated reactive oxygen species production, oxidative phosphorylation, TGFβ interferon signaling similar specific transcription patterns, responses morphological alteration stage infections.

Language: Английский

Citations

0