Manipulation of thebrown glume and internode 1gene leads to alterations in lignified tissue coloration, lignification, and pathogen resistance in wheat DOI Creative Commons
Lei Hua, Rui Song, Xiaohua Hao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 15, 2024

Abstract Lignin is a crucial component of the cell wall, providing mechanical support and protection against biotic abiotic stresses. However, little known about wheat lignin-related mutants their roles in pathogen defense. In this study, we identified an ethyl methanesulfonate (EMS)-derived Aegilops tauschii mutant named brown glume internode 1 ( bgi1 ), which exhibits reddish-brown pigmentation various tissues, including internodes, spikes, glumes. Using map-based cloning single nucleotide polymorphism (SNP) analysis, AET6Gv20438400 BGI1 ) as leading candidate gene, encoding TaCAD1 protein. The mutation occurred splice acceptor site first intron, resulting premature stop codon . We validated function using loss-of-function EMS gene editing knockout mutants, both displayed lignified tissues. exhibited reduced lignin content shearing force relative to wild type, while overexpression transgenic plants showed increased enhanced disease resistance common root rot Fusarium crown rot. confirmed that CAD activity vitro vivo , playing important role biosynthesis. was highly expressed stem spike localized cytoplasm. Transcriptome analysis revealed regulatory networks associated with Finally, demonstrated interacts TaPYL-1D, potentially involved abscisic acid signaling pathway. identification functional characterization significantly advance our understanding proteins biosynthesis plant defense infection wheat.

Language: Английский

Manipulation of the brown glume and internode 1 gene leads to alterations in the colouration of lignified tissues, lignin content and pathogen resistance in wheat DOI Creative Commons
Lei Hua, Rui Song, Xiaohua Hao

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

Summary Lignin is a crucial component of the cell wall, providing mechanical support and protection against biotic abiotic stresses. However, little known about wheat lignin‐related mutants their roles in pathogen defence. Here, we identified an ethyl methanesulfonate (EMS)‐derived Aegilops tauschii mutant named brown glume internode 1 ( bgi1 ), which exhibits reddish‐brown pigmentation various tissues, including internodes, spikes glumes. Using map‐based cloning single nucleotide polymorphism (SNP) analysis, AET6Gv20438400 BGI1 ) as leading candidate gene, encoding TaCAD1 protein. The mutation occurred splice acceptor site first intron, resulting premature stop codon . We validated function using loss‐of‐function EMS gene editing knockout mutants, both displayed lignified tissues. exhibited reduced lignin content shearing force relative to wild type, while overexpression transgenic plants showed increased enhanced disease resistance common root rot Fusarium crown rot. confirmed that CAD activity vitro vivo , playing important role biosynthesis. was highly expressed stem spike, with its localisation observed cytoplasm. Transcriptome analysis revealed regulatory networks associated Finally, demonstrated interacts TaPYL‐1D, potentially involved abscisic acid signalling pathway. identification functional characterisation significantly advance our understanding proteins biosynthesis plant defence infection wheat.

Language: Английский

Citations

0
Manipulation of thebrown glume and internode 1gene leads to alterations in lignified tissue coloration, lignification, and pathogen resistance in wheat DOI Creative Commons
Lei Hua, Rui Song, Xiaohua Hao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 15, 2024

Abstract Lignin is a crucial component of the cell wall, providing mechanical support and protection against biotic abiotic stresses. However, little known about wheat lignin-related mutants their roles in pathogen defense. In this study, we identified an ethyl methanesulfonate (EMS)-derived Aegilops tauschii mutant named brown glume internode 1 ( bgi1 ), which exhibits reddish-brown pigmentation various tissues, including internodes, spikes, glumes. Using map-based cloning single nucleotide polymorphism (SNP) analysis, AET6Gv20438400 BGI1 ) as leading candidate gene, encoding TaCAD1 protein. The mutation occurred splice acceptor site first intron, resulting premature stop codon . We validated function using loss-of-function EMS gene editing knockout mutants, both displayed lignified tissues. exhibited reduced lignin content shearing force relative to wild type, while overexpression transgenic plants showed increased enhanced disease resistance common root rot Fusarium crown rot. confirmed that CAD activity vitro vivo , playing important role biosynthesis. was highly expressed stem spike localized cytoplasm. Transcriptome analysis revealed regulatory networks associated with Finally, demonstrated interacts TaPYL-1D, potentially involved abscisic acid signaling pathway. identification functional characterization significantly advance our understanding proteins biosynthesis plant defense infection wheat.

Language: Английский

Citations

1