Evidence of microglial involvement in the childhood abuse-associated increase in perineuronal nets in the ventromedial prefrontal cortex DOI Creative Commons
Claudia Belliveau, Reza Rahimian, Gohar Fakhfouri

et al.

Brain Behavior and Immunity, Journal Year: 2024, Volume and Issue: 124, P. 321 - 334

Published: Dec. 11, 2024

Microglia, known for their diverse roles in the central nervous system, have recently been recognized involvement degrading extracellular matrix. Perineuronal nets (PNNs), a specialized form of this matrix, are crucial stabilizing neuronal connections and constraining plasticity. Our group reported increased PNN densities ventromedial prefrontal cortex (vmPFC) depressed individuals that died by suicide adulthood after experiencing childhood abuse (DS-CA) compared to matched controls. To explore potential underlying mechanisms, we employed comprehensive approach similar postmortem vmPFC samples, combining human matrix metalloproteinase chemokine array, isolation CD11b-positive microglia enzyme-linked immunosorbent assays (ELISA). findings indicate significant downregulation (MMP)-9 tissue inhibitors metalloproteinases (TIMP)-2 both whole grey matter isolated microglial cells from DS-CA samples. Furthermore, our experiments reveal history child is associated with diminished levels CX3CR1 IL33R lysate CD11b cells. However, ligand, CX3CL1 (Fractalkine), did not differ between groups. While these data suggest long-lasting alterations markers exposed severe adversity, direct functional assessments were conducted. Nonetheless, offer insight into how may contribute via microglial-related mechanisms.

Language: Английский

Postmortem evidence of microglial involvement in the child abuse-associated increase of perineuronal nets in the ventromedial prefrontal cortex DOI Creative Commons
Claudia Belliveau, Reza Rahimian, Gohar Fakhfouri

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 13, 2024

Abstract Microglia, known for their diverse roles in the central nervous system, have recently been recognized involvement degrading extracellular matrix. Perineuronal nets (PNNs), a specialized form of this matrix, are crucial stabilizing neuronal connections and constraining plasticity. Our group reported increased PNN densities ventromedial prefrontal cortex (vmPFC) depressed individuals that died by suicide adulthood after experiencing childhood abuse (DS-CA) compared to matched controls. To explore potential underlying mechanisms, we employed comprehensive approach similar postmortem vmPFC samples, combining human matrix metalloproteinase chemokine array, isolation CD11b-positive microglia enzyme-linked immunosorbent assays (ELISA). findings indicate significant downregulation (MMP)-9 tissue inhibitors metalloproteinases (TIMP)-2 both whole grey matter isolated microglial cells from DS-CA samples. Furthermore, our experiments reveal history child is associated with diminished levels CX3CR1 IL33R lysate CD11b cells. However, ligand, CX3CL1 (Fractalkine), did not differ between groups. While these data suggest long-lasting alterations markers exposed severe adversity, direct functional assessments were conducted. Nonetheless, offer insight into how may contribute via microglial-related mechanisms. Graphical

Language: Английский

Citations

3
Evidence of microglial involvement in the childhood abuse-associated increase in perineuronal nets in the ventromedial prefrontal cortex DOI Creative Commons
Claudia Belliveau, Reza Rahimian, Gohar Fakhfouri

et al.

Brain Behavior and Immunity, Journal Year: 2024, Volume and Issue: 124, P. 321 - 334

Published: Dec. 11, 2024

Microglia, known for their diverse roles in the central nervous system, have recently been recognized involvement degrading extracellular matrix. Perineuronal nets (PNNs), a specialized form of this matrix, are crucial stabilizing neuronal connections and constraining plasticity. Our group reported increased PNN densities ventromedial prefrontal cortex (vmPFC) depressed individuals that died by suicide adulthood after experiencing childhood abuse (DS-CA) compared to matched controls. To explore potential underlying mechanisms, we employed comprehensive approach similar postmortem vmPFC samples, combining human matrix metalloproteinase chemokine array, isolation CD11b-positive microglia enzyme-linked immunosorbent assays (ELISA). findings indicate significant downregulation (MMP)-9 tissue inhibitors metalloproteinases (TIMP)-2 both whole grey matter isolated microglial cells from DS-CA samples. Furthermore, our experiments reveal history child is associated with diminished levels CX3CR1 IL33R lysate CD11b cells. However, ligand, CX3CL1 (Fractalkine), did not differ between groups. While these data suggest long-lasting alterations markers exposed severe adversity, direct functional assessments were conducted. Nonetheless, offer insight into how may contribute via microglial-related mechanisms.

Language: Английский

Citations

0