Developing and characterising bovine decellularized extracellular matrix hydrogels to biofabricate female reproductive tissues DOI Creative Commons

Eduardo Ribes Martínez,

Y. Franko,

Roksan Franko

et al.

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

This study investigated the development and characterization of decellularized extracellular matrix (dECM) hydrogels tailored for biofabrication female reproductive tissues, specifically targeting ovarian cortex, endometrium, medulla, oviduct tissues. We aimed to evaluate cytocompatibility, biomechanical properties, overall efficacy these dECMs in promoting cell viability, proliferation, morphology using bovine model. Bovine species provide a valuable model due their accessibility from slaughterhouse offering practical alternative human samples, which are often limited availability. Additionally, tissue closely mirrors certain physiological biological characteristics humans, making it relevant translational research. Our findings revealed that exhibited high biocompatibility with embryo supporting micro vascularization cellular without need external growth factors. It is important note addition alginate was crucial maintaining structural integrity hydrogel during long-term cultures. These displayed properties mimicked native vital functional activities. The printability assessments showed dECMs, particularly those cortex achieved precision replicating intended structures, though challenges such as low porosity remained. bioprinted constructs demonstrated robust growth, over 97% viability observed by day 7, indicating suitability culture. work represented significant advancement biofabrication, demonstrating potential dECM-based creating structurally viable constructs. By tailoring each dECM match unique different we paved way more effective reliable applications medicine engineering. STATEMENT OF SIGNIFICANCE: research explores use bio-inks Ovarian endometrium essential processes. Notably, microvascularization differentiation supplemental successful bioprinting underscores complex models. represents engineering, promising new avenues medicine.

Language: Английский

Developing and characterising bovine decellularized extracellular matrix hydrogels to biofabricate female reproductive tissues DOI Creative Commons

Eduardo Ribes Martínez,

Y. Franko,

Roksan Franko

et al.

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

This study investigated the development and characterization of decellularized extracellular matrix (dECM) hydrogels tailored for biofabrication female reproductive tissues, specifically targeting ovarian cortex, endometrium, medulla, oviduct tissues. We aimed to evaluate cytocompatibility, biomechanical properties, overall efficacy these dECMs in promoting cell viability, proliferation, morphology using bovine model. Bovine species provide a valuable model due their accessibility from slaughterhouse offering practical alternative human samples, which are often limited availability. Additionally, tissue closely mirrors certain physiological biological characteristics humans, making it relevant translational research. Our findings revealed that exhibited high biocompatibility with embryo supporting micro vascularization cellular without need external growth factors. It is important note addition alginate was crucial maintaining structural integrity hydrogel during long-term cultures. These displayed properties mimicked native vital functional activities. The printability assessments showed dECMs, particularly those cortex achieved precision replicating intended structures, though challenges such as low porosity remained. bioprinted constructs demonstrated robust growth, over 97% viability observed by day 7, indicating suitability culture. work represented significant advancement biofabrication, demonstrating potential dECM-based creating structurally viable constructs. By tailoring each dECM match unique different we paved way more effective reliable applications medicine engineering. STATEMENT OF SIGNIFICANCE: research explores use bio-inks Ovarian endometrium essential processes. Notably, microvascularization differentiation supplemental successful bioprinting underscores complex models. represents engineering, promising new avenues medicine.

Language: Английский

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