Tag with Caution - How protein tagging influences the formation of condensates DOI Creative Commons
Kerstin Dörner, Michelle Gut, Daan Overwijn

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 5, 2024

Summary Fluorescent proteins and peptide tags are essential tools in cellular biology, but can alter the biochemical properties of target proteins. Biomolecular condensates, which have emerged as key principles organization, suggested to provide robustness cells, yet they also respond sensitively small changes environmental conditions—or tagging their components, our findings suggest. Here, we investigated effects sixteen widely used on condensate formation various model organisms, vitro , cells by computational modelling. We find that strongly influenced condensation for some proteins, while others remained unaffected. Effects varied, with enhancing decreasing condensation, depended protein being tagged. Coarse-grained simulations suggest charge fluorescent is a critical factor modulating behavior. Together, results underscore importance rigorous experimental design interpretation experiments.

Language: Английский

Tag with Caution - How protein tagging influences the formation of condensates DOI Creative Commons
Kerstin Dörner, Michelle Gut, Daan Overwijn

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 5, 2024

Summary Fluorescent proteins and peptide tags are essential tools in cellular biology, but can alter the biochemical properties of target proteins. Biomolecular condensates, which have emerged as key principles organization, suggested to provide robustness cells, yet they also respond sensitively small changes environmental conditions—or tagging their components, our findings suggest. Here, we investigated effects sixteen widely used on condensate formation various model organisms, vitro , cells by computational modelling. We find that strongly influenced condensation for some proteins, while others remained unaffected. Effects varied, with enhancing decreasing condensation, depended protein being tagged. Coarse-grained simulations suggest charge fluorescent is a critical factor modulating behavior. Together, results underscore importance rigorous experimental design interpretation experiments.

Language: Английский

Citations

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