FilaBuster: A Strategy for Rapid, Specific, and Spatiotemporally Controlled Intermediate Filament Disassembly DOI Creative Commons

A. T. Moore,

Tommy J. Krug,

Simon B. Hansen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Abstract Intermediate filaments (IFs) play key roles in cellular mechanics, signaling, and organization, but tools for their rapid, selective disassembly remain limited. Here, we introduce FilaBuster, a photochemical approach efficient spatiotemporally controlled IF living cells. FilaBuster uses three-step strategy: (1) targeting HaloTag to IFs, (2) labeling with covalent photosensitizer ligand, (3) light-induced generation of localized reactive oxygen species trigger filament disassembly. This modular strategy applies broadly across subtypes—including vimentin, GFAP, desmin, peripherin, keratin 18—and is compatible diverse dyes imaging platforms. Using vimentin IFs as model system, establish baseline implementation which vimentin-HaloTag labeled ligand triggers rapid specific upon light activation. We then refine this by (i) expanding strategies include nanobody-HaloTag fusion, (ii) broadening the range effective photosensitizers, (iii) optimizing irradiation parameters enable precise spatial control over Together, these findings position robust platform acute, selective, networks, enabling new investigations into structural functional cell physiology disease.

Language: Английский

Versatility of vimentin assemblies: from filaments to biomolecular condensates and back DOI Creative Commons
Dolores Pérez‐Sala, Silvia Zorrilla

European Journal of Cell Biology, Journal Year: 2025, Volume and Issue: unknown, P. 151487 - 151487

Published: April 1, 2025

Language: Английский

Citations

0
FilaBuster: A Strategy for Rapid, Specific, and Spatiotemporally Controlled Intermediate Filament Disassembly DOI Creative Commons

A. T. Moore,

Tommy J. Krug,

Simon B. Hansen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 21, 2025

Abstract Intermediate filaments (IFs) play key roles in cellular mechanics, signaling, and organization, but tools for their rapid, selective disassembly remain limited. Here, we introduce FilaBuster, a photochemical approach efficient spatiotemporally controlled IF living cells. FilaBuster uses three-step strategy: (1) targeting HaloTag to IFs, (2) labeling with covalent photosensitizer ligand, (3) light-induced generation of localized reactive oxygen species trigger filament disassembly. This modular strategy applies broadly across subtypes—including vimentin, GFAP, desmin, peripherin, keratin 18—and is compatible diverse dyes imaging platforms. Using vimentin IFs as model system, establish baseline implementation which vimentin-HaloTag labeled ligand triggers rapid specific upon light activation. We then refine this by (i) expanding strategies include nanobody-HaloTag fusion, (ii) broadening the range effective photosensitizers, (iii) optimizing irradiation parameters enable precise spatial control over Together, these findings position robust platform acute, selective, networks, enabling new investigations into structural functional cell physiology disease.

Language: Английский

Citations

0