Corynebacterium glutamicum: Modellorganismus der bakteriellen Zellbiologie

BIOspektrum, Journal Year: 2025, Volume and Issue: 31(1), P. 9 - 13

Published: Feb. 1, 2025

Language: Английский

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Mapping the ultrastructural topology of the corynebacterial cell surface

PLoS Biology, Journal Year: 2025, Volume and Issue: 23(4), P. e3003130 - e3003130

Published: April 15, 2025

Corynebacterium glutamicum is a diderm bacterium extensively used in the industrial-scale production of amino acids. Corynebacteria belong to bacterial family Mycobacteriaceae , which characterized by highly unusual cell envelope with an outer membrane consisting mycolic acids, called mycomembrane. The mycomembrane further coated surface (S-)layer array C. making this distinctive. Despite biotechnological significance and biomedical mycomembrane-containing pathogens, ultrastructural molecular details its distinctive remain poorly characterized. To address this, we investigated using electron cryotomography cryomicroscopy focused ion beam-milled single dividing cells. Our cellular imaging allowed us map different components onto tomographic density. data reveal that has variable envelope, S-layer decorating patchy manner. We isolated resolved structure at 3.1 Å-resolution particle cryomicroscopy. shows composed hexagonal PS2 protein, interacts directly via anchoring segment containing coiled-coil motif. Bioinformatic analyses revealed sparsely yet exclusively present within genus absent other genera family, suggesting distinct evolutionary pathways development their envelopes. structural collectively provide topography surface, features are shared many pathogenic microbiome-associated bacteria, as well several industrially significant species.

Language: Английский

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A new age in structural S-layer biology - Experimental and in silico milestones

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 110205 - 110205

Published: May 1, 2025

Language: Английский

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