Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cells DOI Creative Commons
Fernando Laguía, Jakub Chojnacki, Itziar Erkizia

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 10, 2025

HIV-1 exploits dendritic cells (DCs) to spread throughout the body via specific recognition of gangliosides present on viral envelope by CD169/Siglec-1 membrane receptor. This interaction triggers internalization within a structure known as sac-like compartment. While mechanism underlying compartment formation remains elusive, prior research indicates that process is clathrin-independent and cell cholesterol-dependent involves transient disruption cortical actin. Here, we investigate potential involvement massive endocytosis (MEND) in this process. We used live confocal imaging measure dimensions dynamics assessed role actin cholesterol fixed using microscopy evaluated effect PI3K protein palmytoilation inhibitors during uptake. Our data demonstrate extensive plasma invagination based (2.9 μm diameter 20 μm3 volume). showed concentration doubles regions uptake, suggesting lipid-phase separation, development accompanied depolarization Moreover, observed palmitoylation inhibition reduce rate from 70% 20% 40%, respectively. results indicate MEND mechanisms formation.

Language: Английский

Massive endocytosis mechanisms are involved in uptake of HIV-1 particles by monocyte-derived dendritic cells DOI Creative Commons
Fernando Laguía, Jakub Chojnacki, Itziar Erkizia

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 10, 2025

HIV-1 exploits dendritic cells (DCs) to spread throughout the body via specific recognition of gangliosides present on viral envelope by CD169/Siglec-1 membrane receptor. This interaction triggers internalization within a structure known as sac-like compartment. While mechanism underlying compartment formation remains elusive, prior research indicates that process is clathrin-independent and cell cholesterol-dependent involves transient disruption cortical actin. Here, we investigate potential involvement massive endocytosis (MEND) in this process. We used live confocal imaging measure dimensions dynamics assessed role actin cholesterol fixed using microscopy evaluated effect PI3K protein palmytoilation inhibitors during uptake. Our data demonstrate extensive plasma invagination based (2.9 μm diameter 20 μm3 volume). showed concentration doubles regions uptake, suggesting lipid-phase separation, development accompanied depolarization Moreover, observed palmitoylation inhibition reduce rate from 70% 20% 40%, respectively. results indicate MEND mechanisms formation.

Language: Английский

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