Integration of cortical inputs in the lateral hypothalamus is dominated by the medial prefrontal cortex DOI Creative Commons

Lotte Razenberg,

P.N. de Greef,

Huibert D. Mansvelder

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

Abstract The lateral hypothalamus (LH) is a critical brain region orchestrating survival behaviours including feeding. Its sparse intrinsic synaptic connectivity allows long-range projections to modulate its activity. Some of these arise from the cerebral cortex, which known influence However, functional and anatomical organization cortico-hypothalamic pathways have remained poorly studied. We used optogenetic mapping show that medial prefrontal cortex (mPFC) strongest cortical input source LH, followed by associative insular (IC), ventral subiculum. Input mPFC IC had markedly different dynamics were integrated supralinearly. surpassed in subpopulation highly excitable dorsal LH neurons strong h-current. showed selective targeting project back mPFC, suggesting existence direct feedback loop. Overall, results identify hypothalamic pathway poised dominate rapid control

Language: Английский

A neural mechanism for learning from delayed postingestive feedback DOI Creative Commons
Christopher A Zimmerman,

Scott S. Bolkan,

Alejandro Pan-Vazquez

et al.

Nature, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Animals learn the value of foods on basis their postingestive effects and thereby develop aversions to that are toxic1-10 preferences those nutritious11-13. However, it remains unclear how brain is able assign credit flavours experienced during a meal with feedback signals can arise after substantial delay. Here we reveal an unexpected role for reactivation neural flavour representations in this temporal credit-assignment process. To begin, leverage fact mice associate novel14,15, but not familiar, delayed gastrointestinal malaise investigate represents support aversive learning. Analyses brain-wide activation patterns network amygdala regions unique being preferentially activated by novel across every stage learning (consumption, memory retrieval). By combining high-density recordings optogenetic stimulation malaise-coding hindbrain neurons, show selectively reactivate from recent meal. The degree malaise-driven individual neurons predicts strengthening responses upon retrieval, which turn leads stabilization population-level representation recently consumed flavour. contrast, degrade absence consequences. Thus, demonstrate plasticity may feedback.

Language: Английский

Citations

2
Brain-wide immunolabeling and tissue clearing applications for engram research DOI
Alessandra Franceschini, Michelle Jin, Claire W. Chen

et al.

Neurobiology of Learning and Memory, Journal Year: 2025, Volume and Issue: unknown, P. 108032 - 108032

Published: Feb. 1, 2025

Language: Английский

Citations

1
Binocular processing facilitates escape behavior through multiple pathways to the superior colliculus DOI
Robin Broersen,

Genevieve Thompson,

F Thomas

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
Combinative protein expression of immediate early genes c-Fos, Arc, and Npas4 along aversive- and reward-related neural networks DOI Creative Commons

Mary Arai,

Hisayuki Osanai,

Chris C. Snell

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Abstract Expression of immediate early genes (IEGs) is critical for memory formation and has been widely used to identify the neural substrate traces, termed engram cells. However, types IEG have relatively not distinguished in identifying cells, as there limited knowledge about extent which different IEGs are selectively or concurrently involved engram. To address this question, we investigated combinative expression c-Fos, Arc, Npas4 proteins using immunohistochemistry following aversive rewarding experiences across subregions prefrontal cortex (PFC), basolateral amygdala (BLA), hippocampal dentate gyrus (DG), retrosplenial (RSC). Using an automated cell detection algorithm, found that patterns Npas4, Arc varied brain areas, with a higher increase expressing cells PFC posterior BLA than DG. The patterns, along their learning-induced changes, also differed areas; co-expression increased learning whereas was less pronounced DG RSC. Furthermore, demonstrate area-to-area functional connectivity networks were extracted by IEGs. These findings provide insights into how combinations will contribute deeper understanding significance IEG-tagged

Language: Английский

Citations

0
Software and pipelines for registration and analyses of rodent brain image data in reference atlas space DOI Creative Commons
Maja Puchades, Sharon C. Yates, Gergely Csucs

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Abstract Advancements in methodologies for large-scale acquisition of high-resolution serial microscopy image data have opened new possibilities experimental studies cellular and subcellular features across whole brains animal models. There is a high demand open-source software workflows automated or semi-automated analysis such data, facilitating anatomical, functional, molecular mapping healthy diseased brains. These share common need to precisely identify, visualize, quantify the location observations within anatomically defined regions, ensuring consistent reproducible interpretation anatomical locations thereby allowing meaningful comparisons results multiple independent studies. Addressing this need, we developed suite desktop web-applications registration brain section images three-dimensional reference atlases (QuickNII, VisuAlign, WebAlign, WebWarp, DeepSlice) performing spatial context provided by an atlas (Nutil, QCAlign, SeriesZoom, LocaliZoom, MeshView). The can be utilized various combinations, creating customized analytical pipelines suited specific research needs. are integrated EBRAINS infrastructure coupled platform, establishing foundation online workbench. We here present our ecosystem, exemplify its use community, discuss possible directions future developments.

Language: Английский

Citations

0
Integration of cortical inputs in the lateral hypothalamus is dominated by the medial prefrontal cortex DOI Creative Commons

Lotte Razenberg,

P.N. de Greef,

Huibert D. Mansvelder

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

Abstract The lateral hypothalamus (LH) is a critical brain region orchestrating survival behaviours including feeding. Its sparse intrinsic synaptic connectivity allows long-range projections to modulate its activity. Some of these arise from the cerebral cortex, which known influence However, functional and anatomical organization cortico-hypothalamic pathways have remained poorly studied. We used optogenetic mapping show that medial prefrontal cortex (mPFC) strongest cortical input source LH, followed by associative insular (IC), ventral subiculum. Input mPFC IC had markedly different dynamics were integrated supralinearly. surpassed in subpopulation highly excitable dorsal LH neurons strong h-current. showed selective targeting project back mPFC, suggesting existence direct feedback loop. Overall, results identify hypothalamic pathway poised dominate rapid control

Language: Английский

Citations

1