Optical Interference for the Guidance of Cryogenic Focused Ion Beam Milling Beyond the Axial Diffraction Limit DOI Creative Commons
Anthony V. Sica, Magda Zaoralová, Cali Antolini

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 3, 2024

Abstract Using a tri-coincident cryogenic FIB-SEM-LM system, we identify an interferometric optical response that can be used for targeting lamella production to fluorescently labelled structures with accuracy beyond the diffraction limit. We demonstrate approach using synthetic samples of fluorescent beads embedded in micron-scale droplets water. then apply capture virions inside host cells. Successful is confirmed by electron tomography revealing clusters intracellular vesicles.

Language: Английский

A structural atlas of death domain fold proteins reveals their versatile roles in biology and function DOI Creative Commons

Evan Wu,

Ankita T. Kandalkar, Julian Ehrmann

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(8)

Published: Feb. 20, 2025

Death domain fold (DDF) superfamily proteins are critically important players in pathways of cell death and inflammation. DDFs often essential scaffolding domains receptors, adaptors, or effectors these by mediating homo- hetero-oligomerization including helical filament assembly. At the downstream ends pathways, effector oligomerization brings enzyme into proximity for their dimerization activation. Hundreds structures have been solved. However, a comprehensive understanding is lacking. In this article, we report curation DDF structural atlas as public website (deathdomain.org) deduce common distinct principles DDF-mediated among four families (death DD, DED, caspase recruitment CARD, pyrin PYD). We further annotate genome-wide based on AlphaFold-predicted models protein sequences. These studies reveal mechanistic rules widely distributed superfamily.

Language: Английский

Citations

0
In situ cryo-electron microscopy and tomography of cellular and organismal samples DOI Creative Commons
Parijat Majumder, Peijun Zhang

Current Opinion in Structural Biology, Journal Year: 2025, Volume and Issue: 93, P. 103076 - 103076

Published: June 4, 2025

Language: Английский

Citations

0
Optical Interference for the Guidance of Cryogenic Focused Ion Beam Milling Beyond the Axial Diffraction Limit DOI Creative Commons
Anthony V. Sica, Magda Zaoralová, Cali Antolini

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 3, 2024

Abstract Using a tri-coincident cryogenic FIB-SEM-LM system, we identify an interferometric optical response that can be used for targeting lamella production to fluorescently labelled structures with accuracy beyond the diffraction limit. We demonstrate approach using synthetic samples of fluorescent beads embedded in micron-scale droplets water. then apply capture virions inside host cells. Successful is confirmed by electron tomography revealing clusters intracellular vesicles.

Language: Английский

Citations

0