Perspective: fluorescence lifetime imaging and single-molecule spectroscopy for studying biological condensates

Methods in microscopy, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Abstract Biological condensates, often formed via liquid-liquid phase separation (LLPS), are membraneless compartments organizing biochemical reactions. Recent advances have shifted the focus from identifying condensates to elucidating their dynamic biological functions, such as buffering concentrations, mediating reactions, and regulating signaling. These critical for cellular processes implicated in diseases like cancer neurodegeneration. Advanced microscopy techniques, including fluorescence lifetime imaging (FLIM), FLIM-FRET, correlation spectroscopy (FCS), enable quantitative, real-time investigations of condensate composition, dynamics, material properties, responses environmental stimuli live cells. This perspective highlights utility time-resolved single-molecule techniques shedding light on interactions with membranes, offering insights into physiology pathology.

Language: Английский

Controlled and orthogonal partitioning of large particles into biomolecular condensates

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 14, 2025

Language: Английский