Hijacking and Integration of Algal Plastids and Mitochondria in a Polar Planktonic Host DOI Creative Commons
Ananya Rao Kedige, Daniel P. Yee, Fabien Chevalier

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

SUMMARY In oceanic plankton, various host organisms are capable of engulfing and temporarily integrating microalgae (photosymbiosis) or just their photosynthetic plastids (kleptoplastidy) as a solar-powered energy source. These cellular interactions can be considered to representative evolutionary steps in plastid acquisition eukaryotes, but the underlying mechanisms dynamics not fully understood. Here, we studied kleptoplastidic dinoflagellate (RSD: Ross Sea Dinoflagellate), which is known steal microalga Phaeocystis antarctica . We tracked morphology activity stolen over several months by combining multimodal subcellular imaging photophysiology. Upon integration inside vacuole, volume pyrenoids significantly increased was boosted along with carbon fixation transfer host. This may supported retention 50-fold larger algal nucleus for ∼1 week. Once lost, there decrease photosynthesis, were still beneficial after > 2 months. Unlike other interactions, showed that mitochondrion also retained months, transforming into an extensive network close proximity plastids. highlights new strategy plankton continuum symbioses where both energy-producing hijacked symbiosis found widely-distributed polar regions suggests plastid-mitochondrion interaction have played role evolution acquisition.

Language: Английский

Visualisation ofEuglena gracilisorganelles and cytoskeleton using expansion microscopy DOI Creative Commons
Anežka Konupková, Priscila Peña‐Diaz, Vladimı́r Hampl

et al.

Life Science Alliance, Journal Year: 2025, Volume and Issue: 8(4), P. e202403110 - e202403110

Published: Feb. 7, 2025

This article explores the use of expansion microscopy, a technique that enhances resolution in fluorescence on autotrophic protist Euglena gracilis . A modified protocol was developed to preserve cell structures during fixation. Using antibodies against key cytoskeletal and organelle markers, α-tubulin, β-ATPase, Rubisco activase, microtubular structures, mitochondria, chloroplasts were visualised. The organisation cytoskeleton corresponded findings from electron microscopy while allowing for visualisation flagellar pocket its entirety revealing previously unnoticed details. study offered insights into shape development mitochondria under varying conditions, such as culture ages light cycles. work demonstrated is robust tool visualising cellular E. , an organism whose internal cannot be stained using standard immunofluorescence because complex pellicle. also serves complement facilitating tomographic reconstructions routine fashion.

Language: Английский

Citations

0
Hijacking and integration of algal plastids and mitochondria in a polar planktonic host DOI Creative Commons
Ananya Rao Kedige, Daniel P. Yee, Fabien Chevalier

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

In oceanic plankton, various hosts are capable of engulfing and temporarily integrating microalgae (photosymbiosis) or just their photosynthetic plastids (kleptoplastidy) from the environment. These cellular interactions have been hypothesized to be representative evolutionary steps in plastid acquisition eukaryotes, but underlying mechanisms not fully understood. Here, we studied a polar kleptoplastidic dinoflagellate, which is known steal microalga Phaeocystis antarctica. We tracked morphology activity stolen over several months by combining multimodal subcellular imaging photophysiology. Upon integration inside host vacuole, volume pyrenoids significantly increased, was boosted. This may supported retention 50-fold larger algal nucleus for ∼1 week. Once lost, there decrease photosynthesis, nucleus- plastid-encoded photosystem subunits were still detected. Carbon fixation transfer also maintained after >2 months. showed that mitochondrion retained months, transforming into an extensive network interacting with plastids. highlights complex strategy plankton along continuum symbioses, where both mitochondria hijacked without nucleus. association, found widely distributed regions, suggests plastid-mitochondrion interaction played role evolution opens new questions about control organelle maintenance.

Language: Английский

Citations

0
Hijacking and Integration of Algal Plastids and Mitochondria in a Polar Planktonic Host DOI Creative Commons
Ananya Rao Kedige, Daniel P. Yee, Fabien Chevalier

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 21, 2024

SUMMARY In oceanic plankton, various host organisms are capable of engulfing and temporarily integrating microalgae (photosymbiosis) or just their photosynthetic plastids (kleptoplastidy) as a solar-powered energy source. These cellular interactions can be considered to representative evolutionary steps in plastid acquisition eukaryotes, but the underlying mechanisms dynamics not fully understood. Here, we studied kleptoplastidic dinoflagellate (RSD: Ross Sea Dinoflagellate), which is known steal microalga Phaeocystis antarctica . We tracked morphology activity stolen over several months by combining multimodal subcellular imaging photophysiology. Upon integration inside vacuole, volume pyrenoids significantly increased was boosted along with carbon fixation transfer host. This may supported retention 50-fold larger algal nucleus for ∼1 week. Once lost, there decrease photosynthesis, were still beneficial after > 2 months. Unlike other interactions, showed that mitochondrion also retained months, transforming into an extensive network close proximity plastids. highlights new strategy plankton continuum symbioses where both energy-producing hijacked symbiosis found widely-distributed polar regions suggests plastid-mitochondrion interaction have played role evolution acquisition.

Language: Английский

Citations

1