Seizure network characterization by functional connectivity mapping and manipulation DOI Creative Commons
James E. Niemeyer,

Peijuan Luo,

Carmen Pons

et al.

Neurophotonics, Journal Year: 2025, Volume and Issue: 12(S1)

Published: Jan. 16, 2025

Despite the availability of various anti-seizure medications, nearly 1/3 epilepsy patients experience drug-resistant seizures. These are left with invasive surgical options that do not guarantee seizure remission. The development novel treatment depends on elucidating complex biology seizures and brain networks. We aimed to develop an experimental paradigm uses anatomical network information, functional connectivity, in vivo models determine how networks, their manipulation, affect propagation. Guided by a known network, we applied widefield calcium imaging neural activity spread through regions, focusing primary somatosensory cortex secondary motor cortex. used microstimulation induce suprathreshold excitatory activation compared this reproducible stimulus acute pharmacologically induced spontaneous In proof-of-concept experiment, ablated single node within bilateral measured effect propagation recruitment. Similar preliminary experiments were repeated chronic model. propagated distinct pattern throughout sequential Seizures recapitulated same pattern, indicating hijacking existing pathways. Ablation key changed contralateral spread. Early cobalt data presented. Here, demonstrate for combining microstimulation, cortical ablation, mapping networks inform patterns can be extended long-term tracking study epileptogenesis other Our findings suggest may useful therapies disorders involving

Language: Английский

Seizure network characterization by functional connectivity mapping and manipulation DOI Creative Commons
James E. Niemeyer,

Peijuan Luo,

Carmen Pons

et al.

Neurophotonics, Journal Year: 2025, Volume and Issue: 12(S1)

Published: Jan. 16, 2025

Despite the availability of various anti-seizure medications, nearly 1/3 epilepsy patients experience drug-resistant seizures. These are left with invasive surgical options that do not guarantee seizure remission. The development novel treatment depends on elucidating complex biology seizures and brain networks. We aimed to develop an experimental paradigm uses anatomical network information, functional connectivity, in vivo models determine how networks, their manipulation, affect propagation. Guided by a known network, we applied widefield calcium imaging neural activity spread through regions, focusing primary somatosensory cortex secondary motor cortex. used microstimulation induce suprathreshold excitatory activation compared this reproducible stimulus acute pharmacologically induced spontaneous In proof-of-concept experiment, ablated single node within bilateral measured effect propagation recruitment. Similar preliminary experiments were repeated chronic model. propagated distinct pattern throughout sequential Seizures recapitulated same pattern, indicating hijacking existing pathways. Ablation key changed contralateral spread. Early cobalt data presented. Here, demonstrate for combining microstimulation, cortical ablation, mapping networks inform patterns can be extended long-term tracking study epileptogenesis other Our findings suggest may useful therapies disorders involving

Language: Английский

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